Gemcitabine is an antimetabolites, which inhibits DNA synthesis with an IC50 of 0.06 µM. Gemcitabine is a nucleoside analogue that is incorporated into replicating DNA, resulting in partial chain termination and stalling of replication forks.
Oncol Lett. 2022 May;23(5):163.
AK4P1 is a cancer-promoting pseudogene in pancreatic adenocarcinoma cells whose transcripts can be transmitted by exosomes
Gemcitabine Hydrochloride purchased from AbMole
Int J Oncol. 2020 Jan;56(1):47-68.
Circulating non‑coding RNA‑biomarker potential in neoadjuvant chemotherapy of triple negative breast cancer?
Gemcitabine Hydrochloride purchased from AbMole
Cell Experiment | |
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Cell lines | BxPC-3, PANC-1 and PaCa-2 cell lines |
Preparation method | Cell Counts. The cells were plated in 6-well plates. Twenty-four hours later, the cells were treated with vehicle, gemcitabine, DMAPT, or the combination. After 72 h of treatment, trypan blue-excluded cell counts were performed in duplicate using a hemocytometer. Cell growth was expressed relative to vehicle-treated cells (100 %). |
Concentrations | 0, 0.005, 0.01, 0.02, 0.04 μM |
Incubation time | 72 h |
Animal Experiment | |
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Animal models | MIA PaCa-2 Xenografts model in athymic nude mice |
Formulation | phosphate-buffered saline, PBS |
Dosages | a single dose of 50 or 100 mg/kg |
Administration | intraperitoneal injection |
Molecular Weight | 299.66 |
Formula | C9H11F2N3O4.HCI |
CAS Number | 122111-03-9 |
Solubility (25°C) | Water 50 mg/mL DMSO 30 mg/mL |
Storage | 2-8°C, protect from light, dry, sealed |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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