Free shipping on all orders over $ 500

Everolimus

Cat. No. M1709

All AbMole products are for research use only, cannot be used for human consumption.

Everolimus Structure
Synonym:

RAD001, SDZ-RAD, Certican

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mM*1mL in DMSO USD 66  USD66 In stock
5mg USD 45  USD45 In stock
10mg USD 66  USD66 In stock
50mg USD 196  USD196 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

Everolimus (RAD001) also known as SDZ-RAD, Certican, Zortress and Afinitorm is an mTOR inhibitor with IC50 of 0.63 nM. Everolimus is an immunosuppressant analog of rapamycin (sirolimus) and inhibits growth factor-mediated proliferation of haematopoietic and nonhaematopoietic cells. Everolimus shows a potent inhibitory effect on posttransplant lymphoproliferative disorder (PTLD)-derived cells in vitro and in vivo.

Product Citations
Customer Product Validations & Biological Datas
Source Breast Cancer Res Treat (2015). Figure 2. Everolimus
Method flow cytometry
Cell Lines KPL-1 cell
Concentrations 10 nM
Incubation Time 5 d
Results Cells were exposed to everolimus at 10 nM for 5 days, and then flow cytometry was performed using Nim- DAPI staining. G0/G1 cell cycle arrest was observed in the two sensitive and the one intermediate cell line tested but was not observed in resistant lines
Source Breast Cancer Res Treat (2015). Figure 1. Everolimus
Method western blot
Cell Lines KPL-1 cell
Concentrations 100 nM
Incubation Time 10 min–48 h
Results We observed a significant inhibition of S6 phosphorylation in all tested cell lines after 30 min exposure to everolimus regardless of sensitivity to growth inhibitory effects.
Protocol (for reference only)
Cell Experiment
Cell lines NCI-N87 and MKN45 cells
Preparation method Proliferation assay. Gastric cancer cells (10,000 cells re-suspended in 1 ml medium) were seeded in 24-well plates. 24 h after seeding,0.5 ml medium containing RAD001 was added to yield desired concentrations of RAD001. At the time points indicated, gastric cancer cells were harvested and counted with a Coulter Z1 electronic cell counter (Beckman Coulter, UK).
Concentrations 2.5–160 n M
Incubation time 72 h
Animal Experiment
Animal models NCI-N87 Tumor xenograft model with Pathogen-free, 4–6 week old, female CB-17 SCID mice
Formulation saline
Dosages 5 mg/kg qd for 121 days
Administration P.O
Chemical Information
Molecular Weight 958.22
Formula C53H83NO14
CAS Number 159351-69-6
Solubility (25°C) DMSO 30 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Manzia et al. Transplant Proc. Everolimus and enteric-coated mycophenolate sodium ab initio after liver transplantation: midterm results.

[2] Caroti et al. Transplant Proc. Conversion from calcineurin inhibitors to everolimus with low-dose cyclosporine in renal transplant recipients with squamous cell carcinoma of the skin.

[3] Papadimitrakopoulou et al. J Thorac Oncol. Everolimus and Erlotinib as Second- or Third-Line Therapy in Patients with Advanced Non-Small-Cell Lung Cancer.

[4] Jensen et al. Am J Cardiol. Comparison of Outcomes in Patients With Versus Without Diabetes Mellitus After Revascularization With Everolimus- and Sirolimus-Eluting Stents (from the SORT OUT IV Trial).

[5] Arora et al. Am J Transplant. Virtual Histology Assessment of Cardiac Allograft Vasculopathy Following Introduction of Everolimus-Results of a Multicenter Trial.

[6] Saito et al. J Invasive Cardiol. Mid-Term Results of Everolimus-Eluting Stent in a Japanese Population Compared With a US Randomized Cohort: SPIRIT III Japan Registry With Harmonization by Doing.

[7] Holdaas et al. Expert Opin Drug Saf. A drug safety evaluation of everolimus in kidney transplantation.

Related mTOR Products
Thioether-cyclized helix B peptide, CHBP

Thioether-cyclized helix B peptide, CHBP can improve metabolic stability and renoprotective effect through inducing autophagy via inhibition of mTORC1 and activation of mTORC2.

10-Hydroxy-2-decenoic acid

10-Hydroxy-2-decenoic acid (10-HDA) is the major lipid component of royal jelly produced by honeybees.

mTOR inhibitor-11

mTOR inhibitor-11 is a brain-penetrant mTOR inhibitor (IC50: 21 nM for pS6).

mTOR inhibitor-12

mTOR inhibitor-12 is a selective brain penetrant mTOR inhibitor without genotoxicity risk.

Royleanone

Royleanone, a diterpenoid isolated from plants, inhibits the proliferation of cancer cells by inducing cell cycle arrest and mitochondria-mediated apoptosis.

  Catalog
Abmole Inhibitor Catalog




Keywords: Everolimus, RAD001, SDZ-RAD, Certican supplier, mTOR, inhibitors, activators

All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.



Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.