Dictamnine (Dictamine) is cytotoxic to human cervical and colon cancer cells, and also has antibacterial and antifungal activities.
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Source | Asian Pac J Cancer Prev (2013). Figure 3. Dictamine |
| Method | Apoptosis assay | |
| Cell Lines | A549 cell line | |
| Concentrations | 300 and 500 μM | |
| Incubation Time | 24 h | |
| Results | However, after exposure to 300 and 500 μM Dic for 24 h, cells with typical morphologic changes of apoptosis that chromatin condensation and shrunken nucleus (Figure 3B and C) were increased. |
 |
Source | Asian Pac J Cancer Prev (2013). Figure 1. Dictamine |
| Method | CCK-8 Assay | |
| Cell Lines | A549 cell line | |
| Concentrations | 100, 300 and 500 μM | |
| Incubation Time | 24 and 48 h | |
| Results | Moreover, the results that A549 cells treated with 300 μM Dic for different times (8, 12, 18 and 24 h) demonstrated that Dic induced A549 cells death in a time-dependent manner |
| Cell Experiment | |
|---|---|
| Cell lines | A549 cell |
| Preparation method | Cell cycle distribution was analyzed using FCM. Briefly, after cells were treated with 300 and 500 μM Dic for 24 h, the cells were harvested, washed twice with PBS, and fixed in 70 % ethanol at 4 °C for 1 h and centrifuged. |
| Concentrations | 300 and 500 μM |
| Incubation time | 24 h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 199.21 |
| Formula | C12H9NO2 |
| CAS Number | 484-29-7 |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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