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Deoxycytidine triphosphate (dCTP), a nucleoside triphosphate, is a raw material in DNA synthesis. Deoxycytidine triphosphate has many applications, such as real-time PCR, cDNA synthesis, and DNA sequencing. dCTP is produced from two sources, the reduction of circulating cytidine-DP (CDP) to form dCDP, which is then phosphorylated to dCTP (the de novo pathway), and, the sequential phosphorylation of dC to dCTP (the dC salvage pathway).
| Cell Experiment | |
|---|---|
| Cell lines | HepG2 cell |
| Preparation method | Extracts were stored at −20°C until determination of dCTP pools by a similar procedure to that described previously. Reaction mixtures (100 μl) contained 0.2μCi [3H]-dATP, 0.25 μm synthetic oligonucleotide primer, 10 mm MgCl2, 5 mm dithiothreitol, 50 mm Tris-HCl (pH 7.5) and 1 unit Sequenase 2.0 enzyme. |
| Concentrations | -- |
| Incubation time | 24 h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 467.16 |
| Formula | C9H16N3O13P3 |
| CAS Number | 2056-98-6 |
| Solubility (25°C) | Water 50 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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