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Colchicine is a natural product extracted from Colchicum spp. It is also a tubulin inhibitor and microtubule disruptor with an IC50 of 3 nM for inhibition of microtubule polymerization, and a competitive antagonist of α3 glycine receptors (GlyRs). Colchicine is also a competitive antagonist of α3 Glycine receptors (GlyRs) and has been used in gout research.
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Source | Cancer Res (2017). Figure 2. Colchicine |
| Method | Scratch migration assay | |
| Cell Lines | A375 and RPMI7951 cells | |
| Concentrations | 25 nM | |
| Incubation Time | 12 and 24 h | |
| Results | The effect of DJ101 on A375 cell colony formation was similar to colchicine at the same concentration, which had colonies covering a total area of 34.7% ± 1.5% |
| Cell Experiment | |
|---|---|
| Cell lines | COS-7 cells |
| Preparation method | After 20 min, the samples were mixed with 5 nM CT20126, colchicine or Taxol and incubated at 37 °C for 5 min. The samples were cooled in an ice bath for depolymerization (still within the light path cell). Successive reactions were performed for another 25 min at 37 °C. |
| Concentrations | 5 nM |
| Incubation time | 5 min |
| Animal Experiment | |
|---|---|
| Animal models | Male Sprague–Dawley rats |
| Formulation | water |
| Dosages | 5 mg/kg |
| Administration | gavage |
| Molecular Weight | 399.44 |
| Formula | C22H25NO6 |
| CAS Number | 64-86-8 |
| Solubility (25°C) | DMSO 45 mg/mL Water 30 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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Fosbretabulin disodium (Combretastatin A-4 phosphate) is a microtubule-targeting agent that binds β-tubulin with Kd of 0.4 μM. |
| Noscapine hydrochloride
Noscapine, a phthalideisoquinoline alkaloid constituting 1–10% of the alkaloid content of opium, has been used as a cough suppressant. |
| Triclabendazole
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| CYT997
CYT997 is a potent microtubule polymerization inhibitor with IC50 of 10-100 nM in cancer cell lines. |
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