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β-Glycerophosphate disodium salt hydrate

Cat. No. M3837
β-Glycerophosphate disodium salt hydrate Structure
Synonym:

BGP; CAS# 154804-51-0

Size Price Availability Quantity
1g USD 30  USD30 In stock
5g USD 62  USD62 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. β-Glycerophosphate disodium salt hydrate is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.

Protocol
Cell Experiment
Cell lines BVSMC bovine vascular smooth muscle cells
Preparation method BVSMCs were cultured in DMEM medium containing 10 mmol/L sodium pyruvate and 15% FBS. After confluence, the cells were seeded in DMEM containing 10 mmol/L sodium pyruvate, 15% FBS, 10 mmol/L β-glycerophosphate, 10-7 mol/L insulin and 50 μg/mL ascorbic acid, and cultured for 14 days (calcification culture) base). The medium was changed every 3 days. In some experiments, only beta-glycerophosphate was used to induce BVSMC calcification. In addition, in the time course experiment, cells were selected as controls before culturing in calcified medium (defined as day 0).
Concentrations 10 mmol/L
Incubation time 14 d
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 306.11
Formula C3H9O6PNa2.5(H2O)
CAS Number 13408-09-8
Purity >99%
Solubility Water 50 mg/mL
Storage 2-8°C, protect from light, dry, sealed
References

[1] Shioi A, et al. Arterioscler Thromb Vasc Biol. Beta-glycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells.

[2] Chung CH, et al. Calcif Tissue Int. Mechanism of action of beta-glycerophosphate on bone cell mineralization.

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  Catalog
Abmole Inhibitor Catalog




Keywords: β-Glycerophosphate disodium salt hydrate, BGP; CAS# 154804-51-0 supplier, Phosphatase, inhibitors

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