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β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. β-Glycerophosphate disodium salt hydrate is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
| Cell Experiment | |
|---|---|
| Cell lines | BVSMC bovine vascular smooth muscle cells |
| Preparation method | BVSMCs were cultured in DMEM medium containing 10 mmol/L sodium pyruvate and 15% FBS. After confluence, the cells were seeded in DMEM containing 10 mmol/L sodium pyruvate, 15% FBS, 10 mmol/L β-glycerophosphate, 10-7 mol/L insulin and 50 μg/mL ascorbic acid, and cultured for 14 days (calcification culture) base). The medium was changed every 3 days. In some experiments, only beta-glycerophosphate was used to induce BVSMC calcification. In addition, in the time course experiment, cells were selected as controls before culturing in calcified medium (defined as day 0). |
| Concentrations | 10 mmol/L |
| Incubation time | 14 d |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 306.11 |
| Formula | C3H9O6PNa2.5(H2O) |
| CAS Number | 13408-09-8 |
| Solubility (25°C) | Water ≥ 80 mg/mL |
| Storage | 2-8°C, protect from light, dry, sealed |
| Related Phosphatase Products |
|---|
| RMC-3943
RMC-3943 is an allosteric SHP2 inhibitor (inhibition of full-length SHP2 in biochemical assay, IC50 = 2.19 nM). |
| LYP-IN-1
LYP-IN-1 is a potent, selective and specific LYP inhibitor with a Ki and an IC50 of 110 nM and 0.259 μM, respectively. |
| PF-07284892
PF-07284892 (ARRY-558) is a potent and orally active SHP2 inhibitor with an IC50 value of 21 nM. |
| MY10
MY10 is a potent and orally active receptor protein tyrosine phosphatase (RPTPβ/ζ) inhibitor. |
| CPDA
CPDA is a novel potent SH2 domain-containing inositol phosphatase 2 (SHIP2) inhibitor. |
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