Basic Blue 20 is a commonly used red-emitting DNA dye with relatively narrow excitation and emission spectra with peaks at 633 nm and 677 nm, respectively, and very high resistance to photobleaching.
| Molecular Weight | 458.47 |
| Formula | C26H33Cl2N3 |
| CAS Number | 82-94-0 |
| Form | Solid |
| Solubility (25°C) | Water 4.72 mg/mL DMSO >100 mg/mL |
| Storage | 4°C, protect from light, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Pooja Murgai et al. Anal Biochem. A flow cytometric cell-cycle assay using methyl green
[4] B Mihaljevic et al. Oncol Rep. Solitary intracranial plasmacytomas
[5] S L HANDLER. Am J Clin Pathol. Automatic trichrome stains
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