Ammonium persulfate substitute (APS substitute) is a product used as a substitute for ammonium persulfate in PAGE and SDS-PAGE gel preparation. The 10% solution prepared with this product is more stable than 10% ammonium persulfate solution when stored at 4℃.
Usage
(1) This product is usually prepared as a 10% aqueous solution and then used to prepare various PAGE gels. 10% Ammonium Persulfate Replacement Aqueous Solution is used in the same way as 10% Ammonium Persulfate in gel preparation.
(2) 10% Ammonium persulfate substitute aqueous solution is less stable at room temperature, and can be stored at 4℃ for 5-7 days and -20℃ for 1-2 months.
| Solubility (25°C) | Water 100 mg/mL |
| Storage | 4°C, dry, sealed |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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