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6-FAM SE

Cat. No. M21712
6-FAM SE Structure
Size Price Availability Quantity
10mg USD 85  USD85 In stock
25mg USD 160  USD160 In stock
50mg USD 260  USD260 In stock
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Quality Control & Documentation
Biological Activity

6-FAM SE is another isomer of carboxyfluorescein. 6-FAM, SE is mainly used in sequencing of nucleic acids and labeling nucleotides.

Chemical Information
Molecular Weight 473.39
Formula C25H15NO9
CAS Number 92557-81-8
Solubility (25°C) DMSO 40 mg/mL
Storage -20°C, protect from light
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Jagpreet S Nanda, et al. Methods Enzymol. Labeling a protein with fluorophores using NHS ester derivitization

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