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5-IAF

Cat. No. M30398
5-IAF Structure
Synonym:

5-Iodoacetamidofluorescein

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Biological Activity

5-IAF (5-Iodoacetamidofluorescein) is an idoacetamide derivate of fluoresceine. 5-IAF can be used as fluorescent probe that labels proteins and other molecules having free thiols (cysteine side chains).

Chemical Information
Molecular Weight 515.25
Formula C22H14INO6
CAS Number 63368-54-7
Form Solid
Solubility (25°C) DMF 100 mg/mL (ultrasonic and warming and heat to 60°C)
DMSO 50 mg/mL (ultrasonic)
Storage -20°C, protect from light
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Salvatore Sotgia, et al. PLoS One. Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection

[2] E H Hellen, et al. Biophys Chem. Nucleotide binding to IAF-labelled Na+/K(+)-ATPase measured by steady state fluorescence quenching by TNP-ADP

[3] S N Muddukrishna, et al. Appl Radiat Isot. Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique

[4] M Steinberg, et al. J Biol Chem. Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein

[5] P A Tyson, et al. J Biol Chem. Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase

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