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7-Amino-4-methylcoumarin-3-acetic acid

Cat. No. M39654
7-Amino-4-methylcoumarin-3-acetic acid Structure
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Biological Activity

7-Amino-4-methylcoumarin-3-acetic acid is a fluorescent protein labelling agent. 7-Amino-4-methylcoumarin-3-acetic acid emits in the blue region (440-460 nm) on activation with UV light (350 nm).

Chemical Information
Molecular Weight 233.22
Formula C12H11NO4
CAS Number 106562-32-7
Form Solid
Solubility (25°C) DMSO 100 mg/mL (ultrasonic)
Storage 4°C, protect from light
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Gabor Kottra et al. Physiol Rep. Peptide transporter isoforms are discriminated by the fluorophore-conjugated dipeptides β-Ala- and d-Ala-Lys-N-7-amino-4-methylcoumarin-3-acetic acid

[2] H I Stefanova et al. Biochemistry. Reactivity of lysyl residues on the (Ca(2+)-Mg2+)-ATPase to 7-amino-4-methylcoumarin-3-acetic acid succinimidyl ester

[3] M W Wessendorf et al. Neurosci Lett. Simultaneous observation of fluorescent retrogradely labeled neurons and the immunofluorescently labeled fibers apposing them using Fluoro-Gold and antisera labeled with the blue fluorochrome 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

[4] J P Aubry et al. J Immunol Methods. 7-amino-4-methylcoumarin-3-acetic acid-conjugated streptavidin permits simultaneous flow cytometry analysis of either three cell surface antigens or one cell surface antigen as a function of RNA and DNA content

[5] M W Wessendorf et al. J Histochem Cytochem. A spectrophotometric method for determination of fluorophore-to-protein ratios in conjugates of the blue fluorophore 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

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