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Cat. No. M1755
WYE-354 Structure
Size Price Availability Quantity
Free Sample 0.5 mg  USD 0 In stock
2mg USD 35  USD35 In stock
5mg USD 50  USD50 In stock
10mg USD 90  USD90 In stock
25mg USD 180  USD180 In stock
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Quality Control & Documentation
Biological Activity

WYE-354 is a potent and ATP competitive inhibitor of mTOR (IC50 = 5 nM), with significant selectivity over PI3K isofoms (>100-fold). WYE-354 is equally potent against mTORC1 and mTORC2 activities in HEK293 immune complex kinase assays using S6K and Akt as the respective substrate (IC50 <200 nM) and effectively blocks cellular phosphorylation of S6K on T389 and Akt on S473 both in cultures and in a murine xenograft model, resulting in a significant suppression of PC3MM2-derived tumor growth (by 86% on day 7; 50 mg/kg, i.p twice per day) in vivo.

Customer Product Validations & Biological Datas
Source Oncotarget (2015). Figure 1. WYE-354
Method MTS assay
Cell Lines Western blot analysis
Concentrations 1 μM
Incubation Time 24, 48, and 72 h
Results WYE-354 significantly reduced cell viability starting at a 1 μM concentration after a 24 hours exposure, in both studied cell lines
Protocol (for reference only)
Cell Experiment
Cell lines Cell lines of Rat1, HEK293, MDA-MB-361, MDA-MB-468, MDA-MB-231, LNCap, DU145, U87MG, A498, HCT116, and HT29
Preparation method Tumor cell growth assays.
Concentrations 0~100 μ M
Incubation time 3 days
Animal Experiment
Animal models PC3MM2 tumors xenograft in BALB/c nu/nu female mice
Formulation formulated in 5% ethanol, 5% polysorbate 80, 5% polyethylene glycol-400
Dosages a single i.p. injection with 50 mg/kg
Administration i.p.
Chemical Information
Molecular Weight 495.5
Formula C24H29N7O5
CAS Number 1062169-56-5
Solubility (25°C) DMSO 90 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Wahdan-Alaswad RS et al. Mol Cancer Res. Inhibition of mTORC1 kinase activates Smads 1 and 5 but not Smad8 in human prostate cancer cells, mediating cytostatic response to rapamycin.

[2] Yu K et al. Cancer Res. Biochemical, cellular, and in vivo activity of novel ATP-competitive and selective inhibitors of the mammalian target of rapamycin.

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Keywords: WYE-354 supplier, mTOR, inhibitors, activators

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