Wnt-C59 (C59) is a PORCN inhibitor for Wnt3A-mediated activation of a multimerized TCF-binding site driving luciferase with IC50 of 74 pM.
|Source||Stem Cells Transl Med (2016). Figure 4. Wnt-C59|
|Cell Lines||human iPSCs|
|Incubation Time||4 or 5 days|
|Results||qPCR analyses revealed that neural aggregates differentiated with C59 contained more cells with rostral identity compared with DKK1 or XAV|
|Cell lines||NMuMG (NMG) and MDA-MB 231 cells|
|Preparation method||Cells are seeded at a concentration of 4×103/well in 100 μL culture medium containing various amouts of Wnt-C59 and incubated for 48 hours at 37 ℃. Add 10 μL/well cell proliferation reagent and incubate for 4 hours at 37 ℃. Shake thoroughly for 1 min on a shaker. Measure the absorbance of the samples in a microplate (ELISA) reader.|
|Incubation time||48 hours|
|Animal models||Female nude mice orthotopically transplanted with independent MMTV-WNT1 tumors|
|Formulation||0.5% methylcellulose and 0.1% Tween-80|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
|Solubility||DMSO 56 mg/mL|
 Jaewoong Jang, et al. Wnt-Signaling Inhibitor Wnt-C59 Suppresses the Cytokine Upregulation in Multiple Organs of Lipopolysaccharide-Induced Endotoxemic Mice via Reducing the Interaction between β-Catenin and NF-κB
|Related Wnt/beta-catenin Products|
Tegatrabetan (BC2059) is a β-Catenin antagonist. Tegatrabetan disrupts the binding of β-catenin with the scaffold protein transducin β-like 1 (TBL1).
CWP232228, a highly potent selective Wnt/β-catenin signaling inhibitor, antagonizes binding of β-catenin to T-cell factor (TCF) in the nucleus.
β-catenin-IN-2 is a potent β-catenin inhibitor, compound H1B1, extracted from patent US20150374662A1. β-catenin-IN-2 can be used for the study of colorectal cancer.
YB-0158 (Wnt pathway inhibitor 2) is a reverse-turn peptidomimetic and a potent colorectal cancer stem cell (CSC) targeting agent. YB-0158 disrupts Sam68-Src interactions and induces apoptosis in CRC cells. Anti-cancer activities.
Pyrvinium pamoate is an FDA-approved antihelmintic drug that inhibits WNT pathway signaling.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.