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TWS119

Cat. No. M2304
TWS119 Structure
Size Price Availability Quantity
5mg USD 48  USD48 In stock
10mg USD 85  USD85 In stock
25mg USD 200  USD200 In stock
50mg USD 320  USD320 In stock
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Quality Control & Documentation
Biological Activity

TWS119 is a glycogen synthase kinase-3β inhibitor with an IC50 of 30 nM. TWS119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells (ESCs) at 400 nM.

Product Citations
Customer Product Validations & Biological Datas
Source Molecular Cancer Research (2018). Figure 5. TWS119 (AbMole BioScience, Houston, TX, USA)
Method inactivate glycogen synthase kinase 3β
Cell Lines CL1-5 cells
Concentrations 50 nM
Incubation Time 18 h
Results In the scrambled control cells, treatment of CL1-5 cells with TWS119 did not affect the basal expression of EMT markers, Slug, and the phosphorylation of Ser9 of GSK3β
Source Exp Cell Res (2018). Figure 1. TWS119
Method CFSE proliferation assay
Cell Lines γδT cells
Concentrations 2 μM
Incubation Time 72 h
Results CFSE dilution assays showed that treatment of PBMCs with TWS119 (2 μM) could simulate γδT cells to divide robustly and with less cell apoptosis compared with the control group
Protocol (for reference only)
Cell Experiment
Cell lines megakaryocytes (MKs)
Preparation method To examine the inhibitory effects of p38MAPK, PI3K, and GSK-3β on MK differentiation and platelet production, hBMMNCs were cultured in MKLI media in the presence or absence (+/−) of each of the following inhibitors for 12 days: 5 µM SB202190 (AbMole, Houston, TX, USA) to inhibit p38MAPK; 10 µM LY294002 (AbMole, Houston, TX, USA) to inhibit PI3K; and 1 µM TWS119 (AbMole, Houston, TX, USA) to inhibit GSK-3β. To investigate the effect of GSK-3β inhibition on platelet production from mature MKs defined as CD41 (+)/CD42b (+)/propidium iodide (PI, Sigma) (−), as described later (flow cytometry analysis in the Methods), mature MKs were then cultured in MKLI media with TWS119 (+/−) for 6 days.
Concentrations 0, 0.17, 1, and 3 µM
Incubation time 12 days
Animal Experiment
Animal models Permanent focal cerebral ischemia rats
Formulation 1 % DMSO
Dosages 30 mg/kg
Administration intraperitoneal
Chemical Information
Molecular Weight 318.33
Formula C18H14N4O2
CAS Number 601514-19-6
Solubility (25°C) DMSO 38 mg/mL
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Wang W, et al. Mol Neurobiol. GSK-3β inhibitor TWS119 attenuates rtPA-induced hemorrhagic transformation and activates the Wnt/β-catenin signaling pathway after acute ischemic stroke in rats.

[2] Ono M, et al. Platelets. GSK-3β negatively regulates megakaryocyte differentiation and platelet production from primary human bone marrow cells in vitro.

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  Catalog
Abmole Inhibitor Catalog




Keywords: TWS119 supplier, GSK-3, inhibitors, activators


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