Free shipping on all orders over $ 500


Cat. No. M2304
TWS119 Structure
Size Price Availability Quantity
10mg USD 90 In stock
25mg USD 200 In stock
50mg USD 345 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
Biological Activity

TWS119 is a glycogen synthase kinase-3β inhibitor with an IC50 of 30 nM. TWS119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells (ESCs) at 400 nM.

Product Citations
Customer Product Validations & Biological Datas
Source Molecular Cancer Research (2018). Figure 5. TWS119 (AbMole BioScience, Houston, TX, USA)
Method inactivate glycogen synthase kinase 3β
Cell Lines CL1-5 cells
Concentrations 50 nM
Incubation Time 18 h
Results In the scrambled control cells, treatment of CL1-5 cells with TWS119 did not affect the basal expression of EMT markers, Slug, and the phosphorylation of Ser9 of GSK3β
Source Exp Cell Res (2018). Figure 1. TWS119
Method CFSE proliferation assay
Cell Lines γδT cells
Concentrations 2 μM
Incubation Time 72 h
Results CFSE dilution assays showed that treatment of PBMCs with TWS119 (2 μM) could simulate γδT cells to divide robustly and with less cell apoptosis compared with the control group
Cell Experiment
Cell lines megakaryocytes (MKs)
Preparation method To examine the inhibitory effects of p38MAPK, PI3K, and GSK-3β on MK differentiation and platelet production, hBMMNCs were cultured in MKLI media in the presence or absence (+/−) of each of the following inhibitors for 12 days: 5 µM SB202190 (AbMole, Houston, TX, USA) to inhibit p38MAPK; 10 µM LY294002 (AbMole, Houston, TX, USA) to inhibit PI3K; and 1 µM TWS119 (AbMole, Houston, TX, USA) to inhibit GSK-3β. To investigate the effect of GSK-3β inhibition on platelet production from mature MKs defined as CD41 (+)/CD42b (+)/propidium iodide (PI, Sigma) (−), as described later (flow cytometry analysis in the Methods), mature MKs were then cultured in MKLI media with TWS119 (+/−) for 6 days.
Concentrations 0, 0.17, 1, and 3 µM
Incubation time 12 days
Animal Experiment
Animal models Permanent focal cerebral ischemia rats
Formulation 1 % DMSO
Dosages 30 mg/kg
Administration intraperitoneal
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 318.33
Formula C18H14N4O2
CAS Number 601514-19-6
Purity 98.09%
Solubility DMSO 38 mg/mL
Storage at -20°C

GSK-3β inhibitor TWS119 attenuates rtPA-induced hemorrhagic transformation and activates the Wnt/β-catenin signaling pathway after acute ischemic stroke in rats.
Wang W, et al. Mol Neurobiol. 2016 Dec;53(10):7028-7036. PMID: 26671619.

GSK-3β negatively regulates megakaryocyte differentiation and platelet production from primary human bone marrow cells in vitro.
Ono M, et al. Platelets. 2011;22(3):196-203. PMID: 21231855.

Related GSK-3 Products

Indirubin-3'-monoxime is a potent GSK-3β inhibitor with IC50 of 22 nM, and inhibits Cdk5/p35, Cdk1/cyclin-B and Cdk2/cyclin-E with IC50s of 100, 180, 250 nM.


Bikinin is a strong activator of brassinosteroid (BR) signaling.


6-bromoindirubin-3′-oxime (BIO) is a potent, reversible and ATP-competitive GSK-3α/β inhibitor and the first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells.

CHIR 99021 trihydrochloride

CHIR 99021 trihydrochloride is a hydrochloride salt of CHIR 99021; selective GSK-3 inhibitor.


IM-12 is a potent and selective GSK-3β inhibitor with IC50 of 53 nM, which also enhances canonical Wnt signalling.

Abmole Inhibitor Catalog 2017

Keywords: TWS119 supplier, GSK-3, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.