Free shipping on all orders over $ 500

Trypsin (porcine pancreas)

Cat. No. M10184
Trypsin (porcine pancreas) Structure
Size Price Availability Quantity
10g USD 42  USD42 In stock
25g USD 84  USD84 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

Trypsin is a serine protease in the digestive system of human and animals. The main function of this enzyme is to hydrolyze proteins into smaller peptides or even amino acids. Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Product Citations
Chemical Information
Molecular Weight 725.78
Formula C35H47N7O10
CAS Number 9002-07-7
Solubility (25°C) Soluble in water (10 mg/ml)
Soluble in phosphate buffers (10 mg/ml)
Storage 2-8°C, dry, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.


[1] Norelle L Daly, et al. J Biol Chem. Structural insights into the role of the cyclic backbone in a squash trypsin inhibitor

[2] Paul P Geurink, et al. J Med Chem. Incorporation of non-natural amino acids improves cell permeability and potency of specific inhibitors of proteasome trypsin-like sites

[3] Masayuki Shimoda, et al. Cell Transplant. Improvement of porcine islet isolation by inhibition of trypsin activity during pancreas preservation and digestion using α1-antitrypsin

[4] R J Read, et al. Biochemistry. Critical evaluation of comparative model building of Streptomyces griseus trypsin

Related Enzymes & Coenzymes Products
Cipaglucosidase alfa

Cipaglucosidase alfa is a unique human recombinant acidic alpha-glucosidase (GAA) that can be used in studies related to Pompe disease.

PreScission Protease

PreScission Protease is a recombinantly expressed 3C protease of human rhinovirus type 14 with a GST tag in Escherichia coli that specifically recognizes the octapeptide sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro or the core pentapeptide sequence Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and cleaves between Gln and Gly amino acid residues. Pro and enzymatically cleaves between Gln and Gly amino acid residues, commonly used to remove Glutathione S-transferase (GST), His, or other tags from fusion proteins.

DNase I (Bovine Pancreas)

DNase I (Bovine Pancreas) is a bovine pancreas-derived DNase I that plays a key role in the cleavage of extracellular DNA, degrading double-stranded DNA into oligodeoxyribonucleotides with a 5ˊ-phosphate and 3ˊ-OH terminus. It can also degrade single-stranded DNA in the presence of monovalent manganese ions, and can be used in molecular biology research.

Lactate oxidase

Lactate oxidase (LOX) is a group of flavin mononucleotide (FMN)-dependent enzymes that catalyze the oxidation of l-lactate using oxygen as the primary electron acceptor, as well as converting lactate to pyruvate and releasing hydrogen peroxide.

Angiotensin Converting Enzyme (from porcine kidney)

The angiotensin-converting enzyme (ACE) is a dipeptidyl-carboxypeptidase which exists in somatic and testicular isoforms with zinc binding motif HEXXH in their active site. ACE regulates blood pressure through renin-angiotensin system.

Abmole Inhibitor Catalog

Keywords: Trypsin (porcine pancreas) supplier, Enzymes & Coenzymes, inhibitors, activators

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.