Free shipping on all orders over $ 500

Tenovin 6 Hydrochloride

Cat. No. M6241
Tenovin 6 Hydrochloride Structure
Synonym:

Tenovin 2

Size Price Availability Quantity
10mM*1mL in DMSO USD 240  USD240 In stock
2mg USD 90  USD90 In stock
5mg USD 220  USD220 In stock
10mg USD 350  USD350 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
Biological Activity

In vitro: Tenovin-6 inhibits the growth of S. cerevisiae cultures with an IC50 of 30 μM and is more toxic to yeast than the less water-soluble tenovin-1. Tenovin-6 rapidly increases the levels of endogenous K382-Ac p53 in MCF-7 cells. Tenovin-6 (0 to 15 μM) dose dependently increases the level of LC3-II in diverse cell types, and the increase is ATG5/7 dependent. Tenovin-6 treatment also increases the number and intensity of autophagic vesicles with or without the presence of Torin 1, and prevents Torin 1-induced SQSTM1/p62 degradation. Tenovin-6 affects the acidification of autolysosomes and impairs the hydrolytic activity of lysosomes but does not affect the fusion between autophagosomes and lysosomes. That tenovin-6 inhibits autophagy does not correlate with p53 activation and SIRT1/2 inhibition by knockdown or knockout cannot mimic the effect of tenovin-6 on LC3B accumulation. Tenovin-6 (0, 1, 2.5, 5 or 10 μM) potently inhibits cell proliferation in a dose- and time-dependent manner in all OCI-Ly1, DHL-10, U2932, RIVA, HBL1 and OCI-Ly10 cell lines. Tenovin-6 consistently increases LC3B-II level in DLBCL cell lines by inhibiting the classical autophagy pathway, without activating p53, and the increase is independent of SIRT1/2/3 and p53. Tenovin-6 induces apoptosis through the extrinsic cell-death pathway. Tenovin-6 suppresses the growth of UM cells with IC50 of 12.8 μM, 11.0 μM, 14.58 μM and 9.62 μM for 92.1, Mel 270, Omm 1 and Omm 2.3 cells, respectively.

In vivo: Tenovin-6 (50 mg/kg, i.p.) inhibits the growth of tumor in mice.

Protocol
Cell Experiment
Cell lines UM cells
Preparation method UM cells are seeded into each well of 96-well plates (5,000 cells/well) and treated the next day with control or Tenovin-6 in an increasing concentrations from 0 to 20 μM for 68 h, and then MTS is added at 20 μL/well to be read at a wave length of 490 nm, the IC50 is determined by curve fitting of the sigmoidal dose-response curve.
Concentrations 0 to 20 μM
Incubation time 68 h
Animal Experiment
Animal models SCID mice
Formulation cyclodextrin 20% (w/v) and DMSO 10% (v/v)
Dosages 50 mg/kg
Administration i.p.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 491.09
Formula C25H35ClN4O2S
CAS Number 1011301-29-3
Purity 99.66%
Solubility 49 mg/mL in DMSO
Storage at -20°C
References

[1] Yuan H, et al. Cell Death Dis. Tenovin-6 impairs autophagy by inhibiting autophagic flux.

[2] Dai W, et al. Sci Rep. Class III-specific HDAC inhibitor Tenovin-6 induces apoptosis, suppresses migration and eliminates cancer stem cells in uveal melanoma.

Related Sirtuin Products
Nicotinamide riboside

Nicotinamide riboside is a precursor to nadir with oral activity that increases NAD levels and activates SIRT1 and SIRT3. Nicotinamide riboside is a source of vitamin B3 (niacin) that enhances oxidative metabolism and prevents metabolic abnormalities induced by a high-fat diet. Nicotinamide riboside can reduce cognitive degradation in transgenic mouse models of Alzheimer's disease.

SRT 1720

SRT 1720 is a potent activator of the human SIRT1, EC1.5 The value is 0.16 μM and simultaneously inhibits SIRT2 and SIRT3, EC1.5 The values are 37 μM and > 300 μM, respectively.

SRT 1460

SRT 1460 is a potent Sirtuin-1 (SIRT1) agonist EC1.5 The value is 2.9 μM.

CAY10602

CAY10602 is a SIRT1 activator, which suppresses the NF-κB-dependent induction of TNF-α by lipopolysaccharide in THP-1 cells dose-dependently.

SRT2183

SRT2183 is a selective activator of Sirtuin-1 (SIRT1) with EC1.5 value of 0.36 μM.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Tenovin 6 Hydrochloride, Tenovin 2 supplier, Sirtuin, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.