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Recombinant Rat IL-1β (E. coli)

Cat. No. M21094
Recombinant Rat IL-1β (E. coli) Structure
Synonym:

Interleukin-1 Beta; IL1F2

Size Price Availability Quantity
10ug USD 185  USD185 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Interleukin-1β (IL-1β) is a non-secretory pro-inflammatory cytokine produced primarily by activated macrophages as well as neutrophils, epithelial cells and endothelial cells. It has metabolic, physiological, and hematopoietic activities and plays one of the central roles in the regulation of the immune response. both IL-1α and IL-1β bind to the same receptors and have similar but not identical biological properties. Mature rat IL1β shares 90% similarity with cotton mice and mice, and 65%-77% similarity with canine, human and rhesus monkey IL1β.

Measured by a cell proliferation assay using mouse D10S cells, the ED50 is < 10.0 pg/ml, corresponding to a specific activity of > 1.0 × 108 units/mg.


Accession # Q63264

Endotoxin < 0.2 EU/μg

Apparent Molecular Weight: ~17.3 kDa, reducing conditions

Lyophilized after extensive dialysis against PBS.

Product Citations
Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Boonyalit Throngnumchai, et al. Protein Expr Purif. Refolded recombinant major capsid protein (MCP) from Infectious Spleen and Kidney Necrosis Virus (ISKNV) effectively stimulates serum specific antibody and immune related genes response in Nile tilapia (Oreochromis niloticus)

[2] H R Anforth, et al. Eur Cytokine Netw. Biological activity and brain actions of recombinant rat interleukin-1alpha and interleukin-1beta

[3] M Zetterstrm, et al. Eur Cytokine Netw. Interleukin-1-mediated febrile responses in mice and interleukin-1 beta activation of NFkappaB in mouse primary astrocytes, involves the interleukin-1 receptor accessory protein

[4] T Takao, et al. Brain Behav Immun. Modulation of interleukin-1 receptors in the brain-endocrine-immune axis by stress and infection

[5] K R Hejnaes, et al. Biol Chem Hoppe Seyler. The effect of N-terminal extension on the structure and function of human interleukin-1 beta

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Keywords: Recombinant Rat IL-1β (E. coli), Interleukin-1 Beta; IL1F2 supplier, Recombinant Proteins, inhibitors, activators


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