Certificate of Analysis


Product Name: Recombinant Rat IL-1β (E. coli)
Catalog Number: M21094

Physical and Chemical Properties
Form: Lyophilized powder
Solubility: Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.
Storage: Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
Analytical Data
QC Standard: >95%, Endotoxin < 0.2 EU/μg
Product Information
Description: Interleukin-1β (IL-1β) is a non-secreted pro-inflammatory cytokine produced primarily by activated macrophages as well as neutrophils, epithelial cells and endothelial cells. Measured by a cell proliferation assay using mouse D10S cells, the ED50 is < 10.0 pg/ml, corresponding to a specific activity of > 1.0 × 108 units/mg.
Stability and Solubility Advice: Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide.
We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details.