All AbMole products are for research use only, cannot be used for human consumption.
The recombinant mouse HMGB1/Fc is a disulfide-linked homodimer. High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family.
HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis are present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses.
However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis.
Measure by its ability to induce TNF alpha in RAW264.7 cells, the ED50 for this effect is <15 ng/mL.
Accession: P63158
Apparent Molecular Weight: 25-35 KDa, under reducing condition
Endotoxin < 0.1 EU/µg
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Form | Lyophilized powder |
Solubility (25°C) | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
[4] Ben Lu, et al. Nature. Novel role of PKR in inflammasome activation and HMGB1 release
[5] A Tesniere, et al. Oncogene. Immunogenic death of colon cancer cells treated with oxaliplatin
Related Other Proteins Products |
---|
Recombinant Mouse Progranulin Protein (HEK293, C-His)
Progranulin (PGRN) proteins regulate lysosomal function, affecting protein transport, enzymatic activity, and acidification. It degrades mature cathepsin D via cathepsin B. The precursor protein, progranulin, is also called Proepithelin and PC cell-derived growth factor. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. |
Recombinant Human HMGB1 Protein (E. coli)
High mobility group protein B1 is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile, it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte, T cell, dendritic cell activities in inflammatory responses. |
Recombinant Mouse MFGE8 Protein (HEK293, C-6×His)
Mouse MFGE8 Protein contributes to phagocytic removal of apoptotic cells in many tissues. It plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. |
Recombinant Human MFGE8 Protein (Baculovirus-Insect, His-AVI, Biotinylated)
MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. MFG-E8 has opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. |
Recombinant Human HMGB1 Protein (E. coli, C-His)
Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (a.a.) single chain polypeptide. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.