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Recombinant Mouse HMGB1 Protein (E. coli, C-His)

Cat. No. M22496

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Recombinant Mouse HMGB1 Protein (E. coli, C-His) Structure
Synonym:

High-mobility group box 1; HMG-1

Size Price Availability Quantity
5ug USD 140  USD140 In stock
20ug USD 340  USD340 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.1 EU/µg
  • COA
  • MSDS
Biological Activity

The recombinant mouse HMGB1/Fc is a disulfide-linked homodimer. High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family.

HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis are present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses.

However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis.

Measure by its ability to induce TNF alpha in RAW264.7 cells, the ED50 for this effect is <15 ng/mL.


Accession: P63158

Apparent Molecular Weight: 25-35 KDa, under reducing condition

Endotoxin < 0.1 EU/µg

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
References

[1] YaQing Zhan, et al. J Immunol. HMGB1-Mediated Neutrophil Extracellular Trap Formation Exacerbates Intestinal Ischemia/Reperfusion-Induced Acute Lung Injury

[2] Min Zhao, et al. J Exp Clin Cancer Res. YAP promotes autophagy and progression of gliomas via upregulating HMGB1

[3] Zhen Wang, et al. Autophagy. Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation

[4] Ben Lu, et al. Nature. Novel role of PKR in inflammasome activation and HMGB1 release

[5] A Tesniere, et al. Oncogene. Immunogenic death of colon cancer cells treated with oxaliplatin

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Keywords: Recombinant Mouse HMGB1 Protein (E. coli, C-His), High-mobility group box 1; HMG-1 supplier, Other Proteins, inhibitors, activators

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