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Recombinant Mouse HMGB1 Protein (HEK293, hFc Tag)

Cat. No. M22496
Recombinant Mouse HMGB1 Protein (HEK293, hFc Tag) Structure
Synonym:

High-mobility group box 1; HMG-1

Size Price Availability Quantity
50ug USD 905  USD905 In stock
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Quality Control & Documentation
  • Purity: >85%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

The recombinant mouse HMGB1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 475 amino acids and has a calculated molecular mass of 53.3 kDa. High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family.

HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis are present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses.

However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis.

A DNA sequence encoding the mouse HMGB1 (P63158) (Met 1-Glu 215) was fused with the Fc region of human IgG1 at the N-terminus.


Lyophilized from sterile PBS, pH 7.4.

Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O or PBS to 100 μg/ml
Storage Stable for up to twelve months from date of receipt at -20℃ to -80℃
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] YaQing Zhan, et al. J Immunol. HMGB1-Mediated Neutrophil Extracellular Trap Formation Exacerbates Intestinal Ischemia/Reperfusion-Induced Acute Lung Injury

[2] Min Zhao, et al. J Exp Clin Cancer Res. YAP promotes autophagy and progression of gliomas via upregulating HMGB1

[3] Zhen Wang, et al. Autophagy. Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation

[4] Ben Lu, et al. Nature. Novel role of PKR in inflammasome activation and HMGB1 release

[5] A Tesniere, et al. Oncogene. Immunogenic death of colon cancer cells treated with oxaliplatin

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Keywords: Recombinant Mouse HMGB1 Protein (HEK293, hFc Tag), High-mobility group box 1; HMG-1 supplier, Recombinant Proteins, inhibitors, activators


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