Species: Human
Expression system: E. coli
Purity: SDS-PAGE analysis > 95%, HPLC analysis > 95%
Endotoxin: Protein content < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~19.4 kDa, reduced by SDS-PAGE
Storage and stability: Upon receipt, the product remains stable at temperatures below -70°C for up to 6 months. After reconstruction, the product should be stable at 4°C for up to 1 week or at -20°C for up to 3 months. For long-term storage, the addition of a carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: ED50 < 0.5 ng/ml, measured in cell proliferation assay using 3T3 cells, corresponds to > 2.0 × 10 6 units /mg of specific activity. Fibroblast growth factor-4 (FGF-4), also known as K-FGF, is a heparin-bound growth factor in the FGF family. It is identified by its carcinogenic transforming activity. FGF-4 and FGF-3 are located on chromosome 11. FGF-4 and its receptors (FGF-R1C, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF-4 is required for normal limb and heart valve development during embryogenesis.
| Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[3] No authors listed. BioDrugs. FGF-4 gene therapy GENERX--Collateral Therapeutics
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