Erythropoietin (EPO) is a glycoprotein hormone known primarily for its role in erythropoiesis, which is responsible for stimulating the proliferation and differentiation of erythroid progenitor cells. Cfu-e cells can differentiate into red blood cells only in the presence of EPO. The physiological levels of EPO in adult mammals are mainly maintained by the kidneys, while in fetal or newborn mammals, EPO levels are maintained by the liver. EPO also performs a variety of non-hematopoietic activities, including vascularization and smooth muscle proliferation, neuroprotection during hypoxia, and stimulation of certain B cells.
DNA sequence encoding human EPO (NP_000790.2) expressed with a His tag at the C-terminus.
Recombinant human EPO protein contains 172 amino acids with a predicted molecular weight of 19.2kD.
Measured by cell proliferation assay with TF-1 cells, the ED50 of this effect is ≤5 ng/mL.
Endotoxin: < 0.1EU/μg
Nucleic Acids Res. 2022 Aug 12;50(14):7938-7958.
HDAC1 and PRC2 mediate combinatorial control in SPI1/PU.1-dependent gene repression in murine erythroleukaemia
Recombinant Human EPO Protein (HEK293, C-His) purchased from AbMole
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
| Storage | Stored at -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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