Free shipping on all orders over $ 500

Recombinant Human ApoE (Mammalian, C-6His)

Cat. No. M22451
Recombinant Human ApoE (Mammalian, C-6His) Structure
Synonym:

Apolipoprotein E; Apo-E

Size Price Availability Quantity
50ug USD 275  USD275 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
  • Purity: >95%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

Recombinant Human Apolipoprotein E is produced by Mammalian expression system and the target gene encoding Lys19-His317 is expressed with a 6His tag at the C-terminus. ApoE is a structural component of very low density lipoprotein (vLDL) synthesized by the liver and intestinally synthesized chylomicrons. ApoE mediates high affinity binding of chylomicrons and vLDL particles to the LDL receptor, allowing for specific uptake of these particles by the liver, preventing the accumulation of cholesterol rich particles in the plasma. Apolipoprotein E combines with fats (lipids) in the body to form molecules called lipoproteins and Apolipoprotein E is a major component of a specific type of lipoprotein called very low-density lipoproteins (VLDLs).


Accession: P02649

APMol Mass: 39 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 5% Trehalose,5% Mannitol, 0.02% Tween80, pH 8.0.

Chemical Information
Solubility (25°C) Dissolve the lyophilized protein in distilled water.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Shaowei Wang, et al. Mol Neurodegener. Calcium-dependent cytosolic phospholipase A2 activation is implicated in neuroinflammation and oxidative stress associated with ApoE4

[2] Li Li, et al. Int J Mol Sci. ABCA1/ApoE/HDL Signaling Pathway Facilitates Myelination and Oligodendrogenesis after Stroke

[3] Akin Akinc, et al. Mol Ther. Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms

[4] A D Roses, et al. Neurobiol Aging. Cis-acting human ApoE tissue expression element is associated with human pattern of intraneuronal ApoE in transgenic mice

[5] P C Rensen, et al. J Clin Invest. Human recombinant apolipoprotein E redirects lipopolysaccharide from Kupffer cells to liver parenchymal cells in rats In vivo

Related Recombinant Proteins Products
Recombinant Human IL-10 (CHO)

Interleukin-10 (IL-10) is usually secreted as a homodimer and, upon binding to its receptor, inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and Th2 cells.

Recombinant Human IL-5 (CHO)

Interleukin-5 (IL-5) is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors.

Recombinant Human Tau-F Protein (E.coli)

Tau proteins are proteins which contain four Tau/MAP repeats. They promote microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity.

Recombinant Human Tau Protein (E. coli, His Tag)

Tau proteins are proteins that stabilize microtubules. Tau protein is a highly soluble microtubule-associated protein (MAP). In humans, these proteins are mostly found in neurons compared to non-neuronal cells. One of tau's main functions is to modulate the stability of axonal microtubules.

Recombinant Rat IL-23 Protein (HEK293, C-His)

IL-23 (IL-23 alpha & IL-12 beta Heterodimer) Protein is a recombinant protein dimer complex containing rat-derived IL-23 alpha & IL-12 beta Heterodimer protein, expressed by HEK293.

  Catalog
Abmole Inhibitor Catalog




Keywords: Recombinant Human ApoE (Mammalian, C-6His), Apolipoprotein E; Apo-E supplier, Recombinant Proteins, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.