All AbMole products are for research use only, cannot be used for human consumption.
NPPB is a blocker of the outwardly rectifying chloride channel (ORCC) with IC50 of 80 nM. NPPB has been shown to activate the GPR35-Gαi/o and GPR35-Gα16 pathways in HEK293 cells, inducing intracellular calcium mobilization.
Acta Med Okayama. 2022 Aug;76(4):415-421.
5-Nitro-2-(3-phenylpropylamino) Benzoic Acid Inhibits the Proliferation and Migration of Lens Epithelial Cells by Blocking CaMKII Signaling
NPPB purchased from AbMole
Int J Mol Med. 2021 Apr;47(4):59.
5‑Nitro‑2‑(3‑phenylpropylamino) benzoic acid induces apoptosis of human lens epithelial cells via reactive oxygen species and endoplasmic reticulum stress through the mitochondrial apoptosis pathway
NPPB purchased from AbMole
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Source | Int J Mol Med (2021). Figure 2. NPPB (Abmole Bioscience Inc., USA) |
| Method | Mitochondrial membrane potential analysis | |
| Cell Lines | HLECs | |
| Concentrations | 50 µM | |
| Incubation Time | 24 h | |
| Results | JC‑1 aggregated in the mitochondria in the control group, with a ratio of 0.15±0.027, while the NPPB‑treated group exhibited a significantly higher ratio (0.59±0.080) since the monomeric form of JC‑1 was present in the cytosol |
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Source | Int J Mol Med (2021). Figure 1. NPPB (Abmole Bioscience Inc., USA) |
| Method | Cell viability assay | |
| Cell Lines | HLECs | |
| Concentrations | 10, 50, 100 and 200 µM | |
| Incubation Time | 24 h | |
| Results | Fig. 1 shows that NPPB significantly inhibited the viability of HLECs in a dose‑dependent manner, with an IC50 value of 53.36±0.1% (P<0.01 vs. 0 µM). These results suggested that NPPB decreased cell survival rate and inhibited the viability of HLECs. A concentration of 50 µM NPPB was used in subsequent experiments. |
| Molecular Weight | 300.31 |
| Formula | C16H16N2O4 |
| CAS Number | 107254-86-4 |
| Solubility (25°C) | DMSO ≥ 60 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
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| GaTx2
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| Anthracene-9-carboxylic acid
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| T16A(inh)-C01
T16A(inh)-C01 is an inhibitor of TMEM16A (ANO1). T16A(inh)-C01 blocks chloride channel mediated by ANO1 with an IC50 of 8.4 μM, without interfering with calcium signaling. |
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