All AbMole products are for research use only, cannot be used for human consumption.
Monomethyl auristatin E (MMAE; Vedotin) is an antimitotic agent which inhibits cell division by blocking the polymerisation of tubulin. Monomethyl auristatin E (MMAE), linked via the lysosomally cleavable dipeptide, valine-citrulline (vc). Antibody-drug conjugates (ADCs) are designed to combine the exquisite specificity of antibodies to target tumor antigens with the cytotoxic potency of chemotherapeutic drugs. In vivo studies demonstrated that the peptide-linked conjugates induced regressions and cures of established tumor xenografts with therapeutic indices as high as 60-fold.*The compound is unstable in solutions, freshly prepared is recommended
| Cell Experiment | |
|---|---|
| Cell lines | CD30+ Karpas 299 cells |
| Preparation method | Using Alamar Blue dye reduction assay to measure cytotoxicity according to the manufacturer's directions. Briefly, freshly preparing a 40% solution (wt/vol) of Alamar Blue in complete media just before adding cultures . Ninety-two hours after drug exposure, adding Alamar Blue solution to cells to constitute 10% culture volume. Incubating cells for 4 hours, and measuring dye reduction on a Fusion HT fluorescent plate reader (Packard Instruments, Meriden, CT). |
| Concentrations | ~1000 ng/mL |
| Incubation time | 96 h |
| Animal Experiment | |
|---|---|
| Animal models | SCID mice bearing Karpas 299 (ALCL) or L540cy (HD) tumors |
| Formulation | |
| Dosages | 0.36 mg/kg |
| Administration | i.v. |
| Molecular Weight | 717.98 |
| Formula | C39H67N5O7 |
| CAS Number | 474645-27-7 |
| Solubility (25°C) | DMSO >48 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[2] Marie Alt, et al. Enfortumab Vedotin in urothelial cancer
| Related Microtubule Products |
|---|
| CCB02
CCB02 is a selective CPAP-tubulin interaction inhibitor, binding to tubulin and competing for the CPAP binding site of β-tubulin, with an IC50 of 689 nM, and shows potent anti-tumor activity. |
| Lisavanbulin
Lisavanbulin (BAL-101553) is the prodrug of the microtubule targeting agent Avanbulin (BAL 27862). |
| 20-O-Demethyl-AP3
20-O-Demethyl-AP3 is a minor metabolite of Ansamitocin P-3. |
| Lisavanbulin dihydrochloride
Lisavanbulin (BAL-101553) dihydrochloride is the prodrug of the microtubule targeting agent Avanbulin (BAL 27862). |
| Paclitaxel-d5
Paclitaxel-d5 |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
