All AbMole products are for research use only, cannot be used for human consumption.
JNK-IN-8 (JNK Inhibitor XVI) is a potent JNK inhibitor with IC50s of 4.7 nM, 18.7 nM, and 1 nM for JNK1, JNK2, and JNK3, respectively. JNK-IN-8 inhibits c-Jun phosphorylation at submicromolar concentrations in cells. JNK-IN-8 will be broadly useful as a pharmacological probe of JNK-dependent signal transduction.
 |
Source | Chem Biol (2012). Figure 4. JNK-IN-8 |
| Method | Western Blot | |
| Cell Lines | HEK293- IL1R cells | |
| Concentrations | 1.0 μM | |
| Incubation Time | 24 h | |
| Results | JNK-IN-7, JNK-IN-8, and JNK-IN-12 exhibited only on-pathway activity as monitored by inhibition of c-Jun phosphorylation |
| Cell Experiment | |
|---|---|
| Cell lines | MDA-MB-436 and HCC1569 cell lines |
| Preparation method | Cells were plated at 40% confluency in 96-well, flat bottom plates. After being allowed to attach overnight, cells were treated with vehicle JNK-IN-8, lapatinib, or JNK-IN-8 and lapatinib combination for various timepoints. After treatment, MTT was added to a final concentration of 0.5mg/ml from 5mg/ml stock in PBS. Cellular metabolism reduces this tetrazole to insoluble formazan crystals. After four hours of incubation, the media + MTT was gently removed. Formazan crystals were dissolved using DMSO and absorbance at 590nm was read. |
| Concentrations | 5 μM |
| Incubation time | 72 h |
| Animal Experiment | |
|---|---|
| Animal models | MDA-MB-231 xenograft nude mice |
| Formulation | - |
| Dosages | 25mg/kg/day |
| Administration | Intraperitoneal injection |
| Molecular Weight | 507.59 |
| Formula | C29H29N7O2 |
| CAS Number | 1410880-22-6 |
| Solubility (25°C) | DMSO ≥ 35 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
[1] Zhang T, et al. Chem Biol. Discovery of potent and selective covalent inhibitors of JNK.
| Related JNK Products |
|---|
| JIP-1(153-163)
JIP-1(153-163) (TI-JIP) is a peptide inhibitor of c-JNK, based on residues 153-163 of JNK-interacting protein-1 (JIP-1) (Modifications: Phe-11 = C-terminal amide). |
| JNK3 inhibitor-5
JNK3 inhibitor-5 is a potent and selective JNK3 inhibitor with an IC50 of 0.379 nM. |
| JNK-1-IN-1
JNK-1-IN-1 is a JNK-1 inhibitor. |
| JNK2-IN-1
JNK2-IN-1 is a JNK2 inhibitor (Kds: 79.2 μM). |
| JNK-1-IN-2
JNK-1-IN-2 is a JNK-1 inhibitor (IC50: 33.5 nM). |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
