BE(2)-C cells require 25 μM of GC7 Sulfate to reduce cell viability by ~50 %. Exposure to 10 and 100 μM GC7 for 72 h clearly decreases the levels of total retinoblastoma (Rb) and phosphorylated Rb as well as of Cdk4 protein, and increases the levels of p21 protein. GC7 Sulfate induces little cytotoxicity in HCC cells between 0 and 20 μM, while higher concentrations of GC7 Sulfate (50 to 100 μM) significantly inhibit the viability of all five HCC cell lines tested.
| Cell Experiment | |
|---|---|
| Cell lines | MYCN2 (±Dox) and BE(2)-C cells |
| Preparation method | Briefly, after treatment with GC7 Sulfate for 72 h at various concentrations (0.1 to 100 μM) in 96-well plates, cells are directly incubated with the MTS dye reagent for 1 h at 37°C in a 5 % CO2 atmosphere. The absorbance is read at 490 nm using a reader. |
| Concentrations | 0.1 to 100 μM |
| Incubation time | 72 h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 270.35 |
| Formula | C8H22N4O4S |
| CAS Number | 150417-90-6 |
| Solubility (25°C) | Water 10 mM |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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