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Recombinant Human DKK-1(Mammalian,N-8His)

Cat. No. M11533
Recombinant Human DKK-1(Mammalian,N-8His) Structure
Synonym:

Dickkopf-related protein 1;Dickkopf-1;Dkk-1

Size Price Availability Quantity
10ug USD 300  USD300 In stock
50ug USD 800  USD800 In stock
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Quality Control
  • Current batch:
  • Purity >95% , Endotoxin <0.01 EU/ug
  • COA
  • MSDS
Biological Activity

Purity: > 95% as determined by reduced SDS-PAGE.
Endotoxin :LAL determination: less than 0.001 ng/ug (0.01 EU/ug).
Storage: Freeze-dried protein should be stored at < -20°C, but at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.

Biological activity: DickkopF-related protein 1(DKK-1) is a member of the DickkopF family. The protein secreted by DKK1 has two cysteine-rich regions separated by junction regions. It antagonizes typical Wnt signaling by inhibiting lRP5/6 interaction with Wnt and forming ternary complexes with transmembrane protein KREMEN that promote lRP5/6 internalization. In adults, Dkks has been associated with bone formation and bone disease, cancer and Alzheimer's disease.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight
Formula
CAS Number
Purity >95% , Endotoxin <0.01 EU/ug
Solubility It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Storage < -20°C
References

[1] Marcin Czepiel, et al. Arthritis Rheumatol. Down-Regulation of Dkk-1 in Platelets of Patients With Axial Spondyloarthritis

[2] Yuan-Hada He, et al. Arch Gynecol Obstet. Detection of DKK-1 gene methylation in exfoliated cells of cervical squamous cell carcinoma and its relationship with high risk HPV infection

[3] John Henderson, et al. Exp Dermatol. Wnt antagonist DKK-1 levels in systemic sclerosis are lower in skin but not in blood and are regulated by microRNA33a-3p

[4] Jaewoong Jang, et al. Sci Rep. LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974

[5] Libo Liang, et al. Tumour Biol. Preliminary mechanism on the methylation modification of Dkk-1 and Dkk-3 in hepatocellular carcinoma

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Keywords: Recombinant Human DKK-1(Mammalian,N-8His), Dickkopf-related protein 1;Dickkopf-1;Dkk-1 supplier, Cytokines, inhibitors

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