Aprotinin is a competitive serine protease inhibitor. Aprotinin reversibly binds to and blocks the enzymatic active site. Aprotinin inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin.
Activity: 5868KIU/mg (4.51TIU/mg)
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Aprotinin purchased from AbMole
| Cell Experiment | |
|---|---|
| Cell lines | mouse G8-1 and C2C12 skeletal muscle myoblasts |
| Preparation method | Olating mouse G8- 1 myoblasts DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reache approximately 40-50% confluence, adding different protease inhibitors to the culture media and incubating cells overnight. Then switching cells to differentiation-promoting media (DMEM + 10% horse serum ?protease inhibitor) and incubating for 7 days. |
| Concentrations | 2 TIU/ml |
| Incubation time | 12hrs |
| Animal Experiment | |
|---|---|
| Animal models | Male Wistar rats |
| Formulation | physiological saline |
| Dosages | 1.5 mg/kg and 3 mg/kg/h, 3 mg/kg and 6 mg/kg/h up to 5 mg/kg and 10 mg/kg/h ological saline |
| Administration | bolus injection followed by a maintenance infusion(i.p.) |
| Molecular Weight | 6511.48 |
| Formula | C284H432N84O79S7 |
| CAS Number | 9087-70-1 |
| Solubility (25°C) | Water ≥ 50 mg/mL |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related Serine/Threonine Protease Products |
|---|
| HC-7366
HC-7366 is an orally bioavailable and efficacious GCN2 kinase activator. |
| Phe-Pro-Ala-pNA
Phe-Pro-Ala-pNA is a chromogenic substrate of tripeptidyl peptidase. |
| Dansyl-Glu-Gly-Arg-Chloromethylketone
Dansyl-Glu-Gly-Arg-Chloromethylketone is a protease inhibitor, and inhibits serine/threonine proteases. |
| Fluorescent Substrate for Subtillsin
Fluorescent Substrate for Subtillsin can be used to detect the enzyme activity of subtillsin. |
| N-Benzoyl-L-tyrosine p-nitroanilide
N-Benzoyl-L-tyrosine p-nitroanilide is a chymotrypsin substrate. |
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