All AbMole products are for research use only, cannot be used for human consumption.
TMPD dihydrochloride is a membrane tethering compound used to study cytochrome C for its effect on yeast cell death. TMPD dihydrochloride is also a substrate for COMPLEX IV. TMPD (N,N,N′,N′-tetramethyl-para-phenylene-diamine) is used in cell culture and microbiology to differentiate organisms that exhibit cytochrome c oxidase activity and to distinguish between Gram-negative and Gram-positive pathogenic and non-pathogenic bacteria. The concept of TMPD oxidation by microorganisms is widely used and is known as a successful colorimetric indicator for bacterial oxidases, as the radical cation TMPD+˙, formed by oxidation, shows a characteristic deep blue colour. The use of N,N,N cent,N cent-tetramethyl-p-phenylenediamine (TMPD) in high throughput microplate assays of COX activity could become the approach of choice in the screening of potential therapeutics that inhibit COX activity in vivo.
| Cell Experiment | |
|---|---|
| Cell lines | Escherichia coli & Bacillus subtilis |
| Preparation method | Bacteria culture and oxidase test Escherichia coli Bacteria were cultured in 2 × TY liquid microbial growth medium (broth), containing 16 g/L tryptone, 10 g/L yeast extract and 5.0 g/L NaCl. Growth medium was inoculated with bacteria from frozen stocks and incubated in glass culture flasks for 18 h at 37 °C in an incubator shaker. An E. coli suspension of 50 μL was transferred into a new culture flask, containing fresh growth medium. Following incubation for 3 to 4 h at 37 °C, the number of bacteria in solution was determined by optical density (OD) at a wavelength of 600 nm (OD600 of 1.0 = 8 × 108 cells/mL). When an OD600 between 0.4 and 1.8 was reached, bacteria were harvested by centrifugation for 15 min at 3000 rcf and re-suspended in pre-warmed (37 °C) PBS. Bacillus subtilis (strain PY79) Low salt growth medium (broth), containing 10 g/L tryptone, 5.0 g/L yeast extract and 5.0 g/L sodium chloride, was inoculated with cultures from frozen stocks and incubated at 30 °C for 36 h in an incubator shaker. An OD600 was determined to calculate number of bacteria in solution (OD600 of 1.0 = 5 × 108 cells/mL). Bacteria were harvested by centrifugation for 10 min at 300 rcf and re-suspended in pre-warmed (30 °C) PBS. Cultures grown on agar, containing 10 g/L tryptone, 5.0 g/L yeast extract 5.0 g/L sodium chloride and 20 g/L agar, were inoculated onto agar plates and grown at 30 °C for 36 h in a static incubator. For the oxidase test, 200 μL of a 1% (wt) N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD-2HCl) solution was added to 1 mL of cell suspension (1 × 107 cells/μL). Images were taken within 30 s of solution mixture. |
| Concentrations | 200 μL of a 1% (wt) N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD-2HCl) solution |
| Incubation time | - |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 237.17 |
| Formula | C10H18Cl2N2 |
| CAS Number | 637-01-4 |
| Solubility (25°C) | Water ≥ 60 mg/mL DMSO ≥ 10 mg/mL |
| Storage | 4°C, protect from light, dry, sealed |
| Related Products |
|---|
| SB-T-1214
SB-T-1214 is a new-generation taxoid. SB-T-1214 possesses significant activity against colon cancer spheroids induced by and enriched with drug resistant tumorigenic CD133high/CD44high cells and efficiently inhibited expression of the majority of stem cell-related genes. |
| NG-497
NG-497 is a selective human adipose triglyceride lipase (ATGL) inhibitor that targets the enzymatically active patatin-like domain of human ATGL. |
| PCL-PEG-MAL
PCL-PEG-MAL is an amphiphilic block copolymer consisting of polycaprolactone (PCL), polyethylene glycol (PEG) and maleimide (MAL) functional groups. PCL-PEG-MAL is used in targeted delivery systems, surface modification of nanomaterials, functionalisation of biomaterials and biosensing technologies for specific chemical linkages or biomolecular immobilisation. |
| DSPE-PEG-Streptavidin
DSPE-PEG-Streptavidin is an important biocoupling molecule widely used in biomedical research, especially in drug delivery, targeted therapies, immunodiagnostics and biosensors. Through the specific binding between streptavidin and biotin, DSPE-PEG-Streptavidin can help to achieve efficient loading and targeted action in targeted delivery systems. In addition, the molecule is widely used in molecular imaging and bioassays, especially for molecular recognition using the strong affinity between streptavidin and biotin in assay platforms that require high sensitivity and specificity. |
| E7107
E7107 is a first-in-class precursor messenger ribonucleic acid (pre-mRNA) spliceosome inhibitor. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.
