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Thiazovivin

Cat. No. M1856
Thiazovivin Structure
Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
2mg USD 40.5  USD45 In stock
5mg USD 67.5  USD75 In stock
10mg USD 117  USD130 In stock
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Quality Control
Biological Activity

Thiazovivin is a selective small molecule Rho-associated kinase (ROCK) inhibitor. When used in combination with ALK5 inhibitor SB-431542 and MEK inhibitor PD-0325901 , it significantly improves (200-fold) the efficiency of iPSC generation from human fibroblast reprogramming. Thiazovivin also improves the survival of human embryonic stem cells upon trypsinization. More over, by regulating E-cadherin mediated cell-cell interaction, Thiazovivin can protects human ES cells from death in the absence of ECM.

Protocol
Cell Experiment
Cell lines hESCs cells
Preparation method For clonal survival assays, single hESCs were diluted to clonal density and plated onto 96-well Matrigel-coated plate. For lowdensity survival assays, 500 cells were plated onto 96-well Matrigelcoated plate. To visualize hESC colonies, cultures were fixed in 4% paraformaldehyde in phosphate buffered saline (PBS) for 5 min, washed once in PBS, then stained for alkaline phosphatase (ALP) activity as described in the manufacturer’s instructions. ALP positive colonies were counted on an inverted microscope.
Concentrations 2 μM
Incubation time 4 days
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 311.36
Formula C15H13N5OS
CAS Number 1226056-71-8
Purity 99.73%
Solubility DMSO 25 mg/mL
Storage at -20°C
References

Direct conversion of porcine embryonic fibroblasts into adipocytes by chemical molecules.
Zhu et al. Cell Reprogram. 2012 Apr;14(2):99-105. PMID: 22372576.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Thiazovivin supplier, ROCK, inhibitors

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