All AbMole products are for research use only, cannot be used for human consumption.
T7 RNA polymerase is a DNA-dependent RNA polymerase that specifically recognizes the T7 promoter sequence (5' -TaatacgactCactatagggaga-3 '). T7 RNA polymerase can catalyze NTP incorporation of single or double stranded DNA template downstream of T7 promoter to synthesize RNA complementary to the downstream DNA template of T7 promoter. Linear plasmids containing T7 promoters or PCR products containing T7 promoters can be used as templates for RNA synthesis in vitro. It can be used to synthesize various RNA precursors including mRNA, siRNA, gRNA and so on.
Source: This product is expressed by Escherichia coli and the expressed gene is phage T7 RNA polymerase gene
Storage and stability: stored at -20℃, valid for 24 months
Advantages: Highly specific recognition of T7 promoter, no nuclease, no protease
Storage | -20°C |
[1] Luiz F M Passalacqua, et al. RNA. Single-pass transcription by T7 RNA polymerase
[4] S Tabor. Curr Protoc Mol Biol. Expression using the T7 RNA polymerase/promoter system
[5] Rui Sousa, et al. Prog Nucleic Acid Res Mol Biol. T7 RNA polymerase
Related Nucleic Acid Extraction and Purification Products |
---|
RNase A, bovine pancreas
RNase A, bovine pancreas cleaves RNA 3' to pyrimidines and cleaves RNA at each pyrimidine residue.Ribonuclease A catalyzes the hydrolysis of single-stranded RNA in the absence of metal ions or cofactors. |
RNase Inhibitor, Murine
RNase Inhibitor, Murine specifically inhibits RNases A, B and C, thereby protecting RNA from degradation by these enzymes. |
Universal nuclease |
Recombinant DNase I (RNase Free) |
Recombinant RNase R
Recombinant RNase R (E. coli) is a magnesium-dependent 3′→5′ exoribonuclease that can digest all linear RNAs but does not digest lariat or circular RNA structures. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA and intron lariats. |
All AbMole products are for research use only, cannot be used for human consumption or veterinary use. We do not provide products or services to individuals. Please comply with the intended use and do not use AbMole products for any other purpose.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2024 AbMole BioScience. All Rights Reserved.