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T7 RNA Polymerase (E. coli)

Cat. No. M11532

All AbMole products are for research use only, cannot be used for human consumption.

T7 RNA Polymerase (E. coli) Structure
Size Price Availability Quantity
2000U USD 130  USD130 In stock
5000U USD 210  USD210 In stock
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Quality Control & Documentation
Biological Activity

T7 RNA polymerase is a DNA-dependent RNA polymerase that specifically recognizes the T7 promoter sequence (5' -TaatacgactCactatagggaga-3 '). T7 RNA polymerase can catalyze NTP incorporation of single or double stranded DNA template downstream of T7 promoter to synthesize RNA complementary to the downstream DNA template of T7 promoter. Linear plasmids containing T7 promoters or PCR products containing T7 promoters can be used as templates for RNA synthesis in vitro. It can be used to synthesize various RNA precursors including mRNA, siRNA, gRNA and so on.

Source: This product is expressed by Escherichia coli and the expressed gene is phage T7 RNA polymerase gene

Storage and stability: stored at -20℃, valid for 24 months

Advantages: Highly specific recognition of T7 promoter, no nuclease, no protease

Chemical Information
Storage -20°C
References

[1] Luiz F M Passalacqua, et al. RNA. Single-pass transcription by T7 RNA polymerase

[2] Subhomoi Borkotoky, et al. Int J Biol Macromol. The highly efficient T7 RNA polymerase: A wonder macromolecule in biological realm

[3] Donald C Rio. Cold Spring Harb Protoc. Expression and purification of active recombinant T7 RNA polymerase from E. coli

[4] S Tabor. Curr Protoc Mol Biol. Expression using the T7 RNA polymerase/promoter system

[5] Rui Sousa, et al. Prog Nucleic Acid Res Mol Biol. T7 RNA polymerase

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Keywords: T7 RNA Polymerase (E. coli) supplier, Nucleic Acid Extraction and Purification, inhibitors, activators

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