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Streptavidin-FITC

Cat. No. M9939
Streptavidin-FITC Structure
Size Price Availability
1mg USD 400  USD400 4-7 Days
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Quality Control & Documentation
  • Purity: ≥5 units/mg protein
  • COA
  • MSDS
Biological Activity

Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l.

Chemical Information
Storage -20°C, dry, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Tsutomu Tanaka, et al. Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction

[2] Shan Hu, et al. Biotin induced fluorescence enhancement in resonance energy transfer and application for bioassay

[3] Hong Ling Liu, et al. Synthesis of streptavidin-FITC-conjugated core-shell Fe3O4-Au nanocrystals and their application for the purification of CD4+ lymphocytes

[4] Lu Ye, et al. Biotinylation of TiO(2) nanoparticles and their conjugation with streptavidin

[5] Moon Suk Kim, et al. Preparation of a gradient biotinylated polyethylene surface to bind streptavidin-FITC

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