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Rhodamine B hydrazide

Cat. No. M45081
Rhodamine B hydrazide Structure
Size Price Availability Quantity
100mg USD 80  USD80 In stock
250mg USD 135  USD135 In stock
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Quality Control & Documentation
Biological Activity

Rhodamine B hydrazide is a good sulfite probe with colorless, non-fluorescent properties, which produces fluorescence intensity dependent on sulfite concentration (5-800 ng/mL; detection limit = 1.4 ng/mL (3σ)). Sulfite reduction solubilizes oxygen and generates superoxide radicals, which bind to Rhodamine B hydrazide to form the fluorescent product Rhodamine B. This fluorescence can be enhanced by Tween 80 surfactant micelles.Rhodamine B hydrazide exhibits maximum absorption at 554 nm and maximum fluorescence emission at 574 nm. Rhodamine B hydrazide has maximum absorption at 554 nm and maximum fluorescence emission at 574 nm.

Chemical Information
Molecular Weight 456.58
Formula C28H32N4O2
CAS Number 74317-53-6
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

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