Rhodamine B hydrazide is a good sulfite probe with colorless, non-fluorescent properties, which produces fluorescence intensity dependent on sulfite concentration (5-800 ng/mL; detection limit = 1.4 ng/mL (3σ)). Sulfite reduction solubilizes oxygen and generates superoxide radicals, which bind to Rhodamine B hydrazide to form the fluorescent product Rhodamine B. This fluorescence can be enhanced by Tween 80 surfactant micelles.Rhodamine B hydrazide exhibits maximum absorption at 554 nm and maximum fluorescence emission at 574 nm. Rhodamine B hydrazide has maximum absorption at 554 nm and maximum fluorescence emission at 574 nm.
| Molecular Weight | 456.58 |
| Formula | C28H32N4O2 |
| CAS Number | 74317-53-6 |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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