Certificate of Analysis
| Product Name: | Rhodamine B hydrazide |
| Catalog Number: | M45081 |
| CAS Number: | 74317-53-6 |
Physical and Chemical Properties
| Formula: | C28H32N4O2 |
| Molecular Weight: | 456.58 |
| Storage: |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Chemical Structure: |  |
Analytical Data
| QC Standard: | >98.0% |
| H-NMR: | Consistent with structure |
Product Information
| Description: | Rhodamine B hydrazide is a good sulfite probe with colorless, non-fluorescent properties, which produces fluorescence intensity dependent on sulfite concentration (5-800 ng/mL; detection limit = 1.4 ng/mL (3σ)). Sulfite reduction solubilizes oxygen and generates superoxide radicals, which bind to Rhodamine B hydrazide to form the fluorescent product Rhodamine B. This fluorescence can be enhanced by Tween 80 surfactant micelles.Rhodamine B hydrazide exhibits maximum absorption at 554 nm and maximum fluorescence emission at 574 nm. Rhodamine B hydrazide has maximum absorption at 554 nm and maximum fluorescence emission at 574 nm. |
| Stability and Solubility Advice: | Information concerning product stability, particularly in solution, has rarely been reported and in most cases we can only offer a general guide. We recommend that stock solutions, once prepared, are stored aliquoted in tightly sealed vials and used within 1 month. Avoid repeated freeze and thaw cycles. Storage conditions for some special products should refer to their storage details. |