Expression system: P. pastoris
Purity: SDS-PAGE > 95%
Endotoxin level: protein < 0.5 EU/μg by gel coagulation
Apparent molecular weight: ~ 38-40kDa, SDS-PAGE under non-reduction conditions
~ 18-20kDa, under sdS-PAGE reduction condition
Formulation: Lyophilized after extensive dialysis against 25 mM HEPES, 150 mM NaCl, pH 7.0.
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Biological activity: Vascular endothelial growth factor (VEGF)（P. pastoris) is an effective growth and angiogenesis cytokine. Stimulates endothelial cell proliferation and survival, promotes angiogenesis and vascular permeability. Vascular endothelial growth factor (VEGF) is expressed in vascularized tissues and plays an important role in normal and pathological angiogenesis. There is considerable evidence that vascular endothelial growth factor (VEGF) plays an important role in tumor metastasis and induction of intraocular neovascularization syndrome. Vascular endothelial growth factor (VEGF) signals are transmitted through three receptors; Fms-like tyrosine kinase (FLT-1), KDR gene products (the homolog of mouse KDR is FLK-1 gene products) and FLT4 gene products.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml |
| Storage | Stable for up to 6 months at lower than -70°C after receipt |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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