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Recombinant Human VEGF165 (P. pastoris)

Cat. No. M11490

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Human VEGF165 (P. pastoris) Structure
Synonym:

VEGF-165; Vascular Endothelial Growth Factor 165; VPF

Size Price Availability Quantity
10ug USD 188  USD188 In stock
50ug USD 540  USD540 In stock
100ug USD 850  USD850 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.5 EU/μg
  • COA
  • MSDS
Biological Activity

Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.

Recombinant Human VEGF165 expressed the target gene encoding Ala27-Arg191.

Measured by the dose-dependent stimulation of the proliferation of HUVEC cells, the ED50 is of 1.0-5.0 ng/ml, corresponding to a specific activity of 2.0 × 105-1.0 × 106 units/mg.


Accession: P15692-4

Endotoxin < 0.5 EU/µg

Apparent Molecular Weight: ~ 18-20 kDa, under reducing conditions

Lyophilized after extensive dialysis against 25 mM HEPES, 150 mM NaCl, pH 7.0.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
References

[1] Weijie Zhou, et al. Sheng Wu Gong Cheng Xue Bao. [Production of high-purity recombinant human vascular endothelial growth factor (rhVEGF165) by Pichia pastoris]

[2] Kinjal A Patel, et al. J Obstet Gynaecol Res. Overexpression of VEGF165 is associated with poor prognosis of cervical cancer

[3] Tianyang Zhang, et al. Transgenic Res. Expression and characterization of recombinant human VEGF165 in the middle silk gland of transgenic silkworms

[4] Zhaohui He, et al. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. [VEGF165 transfected endothelial progenitor cells mediated by lentivirus alleviated ALI in rats]

[5] Lei Ye, et al. Exp Mol Med. High efficiency transduction of human VEGF165 into human skeletal myoblasts: in vitro studies

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Keywords: Recombinant Human VEGF165 (P. pastoris), VEGF-165; Vascular Endothelial Growth Factor 165; VPF supplier, Cytokines and Growth Factors, inhibitors, activators

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