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Recombinant Streptococcus pyogenes CRISPR-Cas9 Protein (Baculovirus-Insect, His)

Cat. No. M25476
Recombinant Streptococcus pyogenes CRISPR-Cas9 Protein (Baculovirus-Insect, His) Structure
Size Price Availability Quantity
20ug USD 950  USD950 In stock
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Quality Control & Documentation
  • Purity: >90%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

The recombinant s. pyogenes serotype M1 cas9 consists of 1407 amino acids and predicts a molecular mass of 163 kDa.

A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.


Activity: 1 ug = 5 U; 1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37°C

Endotoxin: < 1.0 EU per μg protein

Formulation: Liquid. Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP.

Chemical Information
Storage Stable for up to twelve months from date of receipt at -20℃ to -80℃
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Gabriela Pannunzio Carmignotto, et al. J Biotechnol. On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains

[2] A V Bannikov, et al. Mol Biol (Mosk). [CRISPR/CAS9, the King of Genome Editing Tools]

[3] Shahad Albadri, et al. Methods. Genome editing using CRISPR/Cas9-based knock-in approaches in zebrafish

[4] Jian-Hua Zhang, et al. Bioengineered. Optimization of genome editing through CRISPR-Cas9 engineering

[5] Le Cong, et al. Methods Mol Biol. Genome engineering using CRISPR-Cas9 system

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