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Recombinant Rat TNF-α (P. pastoris)

Cat. No. M21466
Recombinant Rat TNF-α (P. pastoris) Structure
Synonym:

TNF-alpha; Tumor necrosis factor ligand superfamily member 2

Size Price Availability Quantity
10ug USD 155  USD155 In stock
50ug USD 440  USD440 In stock
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Quality Control
  • Current batch:
  • Purity >95%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Species: Rat
Expression system: P. pastoris
Purity: SDS-PAGE >95%, HPLC >95%
Endotoxin: Determination of <0.2 EU/μg protein by gel coagulation
Apparent molecular weight: about 17.4kDa on SDS-PAGE under reduced condition
Storage and stability: Upon receipt, the product remains stable at temperatures below -70°C for up to 6 months. After reconstruction, the product should be stabilized at 4°C for 1 week or at -20°C for 3 months. For long-term storage, the addition of a carrier protein (e.g. 0.1%BSA) is recommended. Avoid repeated freeze-thaw cycles.


Biological activity: Tumor necrosis factor-α (TNF-α) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. In addition to inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, rheumatoid arthritis, and graft-versus-host disease. Tnf-1a is a potent lymphofactor that has cytotoxic effects on a variety of tumor cells and some other target cells.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight
Formula
CAS Number
Purity >95%, Endotoxin < 0.2 EU/μg
Solubility It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage at -20°C
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Keywords: Recombinant Rat TNF-α (P. pastoris), TNF-alpha; Tumor necrosis factor ligand superfamily member 2 supplier, Cytokines, inhibitors

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