Free shipping on all orders over $ 500

Recombinant Rat TNF-α (P. pastoris)

Cat. No. M21466
Recombinant Rat TNF-α (P. pastoris) Structure
Synonym:

TNF-alpha; Tumor necrosis factor ligand superfamily member 2

Size Price Availability Quantity
10ug USD 155  USD155 In stock
50ug USD 440  USD440 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Tumor necrosis factor-α (TNF-α) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. In addition to inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, rheumatoid arthritis, and graft-versus-host disease. Tnf-1a is a potent lymphofactor that has cytotoxic effects on a variety of tumor cells and some other target cells. 


Accession #: P06804 

Apparent molecular weight: about 17.4kDa on SDS-PAGE under reduced condition 

Endotoxin: < 0.2 EU/μg 

Lyophilized after extensive dialysis against PBS.

Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.
Storage Stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Mingde Ji, et al. PLoS One. C5a Induces the Synthesis of IL-6 and TNF-α in Rat Glomerular Mesangial Cells through MAPK Signaling Pathways

[2] Xu-Hong Wei, et al. Exp Neurol. Peri-sciatic administration of recombinant rat TNF-alpha induces mechanical allodynia via upregulation of TNF-alpha in dorsal root ganglia and in spinal dorsal horn: the role of NF-kappa B pathway

[3] G S Rees, et al. Eur Cytokine Netw. Rat tumour necrosis factor-alpha: expression in recombinant Pichia pastoris, purification, characterization and development of a novel ELISA

[4] A K Olejnik, et al. Immunol Lett. Tumor necrosis factor alpha (TNF-alpha) modulates rat mast cell reactivity

[5] P M Hopkins. Intensive Care Med. Human recombinant TNF alpha affects rat diaphragm muscle in vitro

Related Recombinant Proteins Products
Recombinant Human Tau-F Protein (E.coli)

Tau proteins are proteins which contain four Tau/MAP repeats. They promote microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity.

Recombinant Human Tau Protein (E. coli, His Tag)

Tau proteins are proteins that stabilize microtubules. Tau protein is a highly soluble microtubule-associated protein (MAP). In humans, these proteins are mostly found in neurons compared to non-neuronal cells. One of tau's main functions is to modulate the stability of axonal microtubules.

Recombinant Rat IL-23 Protein (HEK293, C-His)

IL-23 (IL-23 alpha & IL-12 beta Heterodimer) Protein is a recombinant protein dimer complex containing rat-derived IL-23 alpha & IL-12 beta Heterodimer protein, expressed by HEK293.

Recombinant Rat IL-6 (HEK293)

Interleukin-6 (IL-6), also known as BSF-2, CDF and IFNB2, is a pleiotropic cytokine that participates in both pro-inflammatory and anti-inflammatory responses.

Recombinant Rat IL-12 (Mammalian, C-6His)

Interleukin 12 (IL-12) has important immunological functions. IL12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells.

  Catalog
Abmole Inhibitor Catalog




Keywords: Recombinant Rat TNF-α (P. pastoris), TNF-alpha; Tumor necrosis factor ligand superfamily member 2 supplier, Recombinant Proteins, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.