Expression system: P. pastoris
Purity: SDS-PAGE >95%, HPLC >95%
Endotoxin: Determination of <0.2 EU/μg protein by gel coagulation
Apparent molecular weight: about 17.4kDa on SDS-PAGE under reduced condition
Storage and stability: Upon receipt, the product remains stable at temperatures below -70°C for up to 6 months. After reconstruction, the product should be stabilized at 4°C for 1 week or at -20°C for 3 months. For long-term storage, the addition of a carrier protein (e.g. 0.1%BSA) is recommended. Avoid repeated freeze-thaw cycles.
Biological activity: Tumor necrosis factor-α (TNF-α) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. In addition to inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, rheumatoid arthritis, and graft-versus-host disease. Tnf-1a is a potent lymphofactor that has cytotoxic effects on a variety of tumor cells and some other target cells.
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
|Purity||＞95%, Endotoxin < 0.2 EU/μg|
|Solubility||It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.|
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