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Recombinant Rat TNF-α (P. pastoris)

Cat. No. M21466
Recombinant Rat TNF-α (P. pastoris) Structure
Synonym:

TNF-alpha; Tumor necrosis factor ligand superfamily member 2

Size Price Availability Quantity
10ug USD 155  USD155 In stock
50ug USD 440  USD440 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Tumor necrosis factor-α (TNF-α) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. In addition to inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, rheumatoid arthritis, and graft-versus-host disease. Tnf-1a is a potent lymphofactor that has cytotoxic effects on a variety of tumor cells and some other target cells. 


Accession #: P06804 

Apparent molecular weight: about 17.4kDa on SDS-PAGE under reduced condition 

Endotoxin: < 0.2 EU/μg 

Lyophilized after extensive dialysis against PBS.

Product Citations
Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml.
Storage Stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Mingde Ji, et al. PLoS One. C5a Induces the Synthesis of IL-6 and TNF-α in Rat Glomerular Mesangial Cells through MAPK Signaling Pathways

[2] Xu-Hong Wei, et al. Exp Neurol. Peri-sciatic administration of recombinant rat TNF-alpha induces mechanical allodynia via upregulation of TNF-alpha in dorsal root ganglia and in spinal dorsal horn: the role of NF-kappa B pathway

[3] G S Rees, et al. Eur Cytokine Netw. Rat tumour necrosis factor-alpha: expression in recombinant Pichia pastoris, purification, characterization and development of a novel ELISA

[4] A K Olejnik, et al. Immunol Lett. Tumor necrosis factor alpha (TNF-alpha) modulates rat mast cell reactivity

[5] P M Hopkins. Intensive Care Med. Human recombinant TNF alpha affects rat diaphragm muscle in vitro

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Keywords: Recombinant Rat TNF-α (P. pastoris), TNF-alpha; Tumor necrosis factor ligand superfamily member 2 supplier, Recombinant Proteins, inhibitors, activators


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