Free shipping on all orders over $ 500

Recombinant Rat IL-10 (CHO)

Cat. No. M21121
Recombinant Rat IL-10 (CHO) Structure
Synonym:

B-TCGF; CSIF; TGIF

Size Price Availability Quantity
10ug USD 208  USD208 In stock
50ug USD 640  USD640 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Interleukin-10 (IL-10) is produced by many immune cells, such as T cells, macrophages, mast cells and dendritic cells. It is usually secreted as a homodimer and, upon binding to receptors, inhibits the synthesis of several cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and Th2 cells. It also displays the ability to suppress Antigen-Presenting Cell (APC) function.

Measured in a bioassay using C6 cells, the ED50 is < 8.0 μg /ml.


Protein Construction: IL-10 (Ser19-Asn178)

Accession#: P29456

Apparent Molecular Weight: 8~22 kDa, reducing conditions

Endotoxin < 0.2 EU/μg

Lyophilized after extensive dialysis against PBS.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for up to 6 months after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Yunxia Liu, et al. Toxins (Basel). Study of Anti-Inflammatory and Analgesic Activity of Scorpion Toxins DKK-SP1/2 from Scorpion Buthus martensii Karsch ( Bm K)

[2] Daewook Kim, et al. Mol Ther Methods Clin Dev. AAV-Mediated Combination Gene Therapy for Neuropathic Pain: GAD65, GDNF, and IL-10

[3] Cristine Steen-Louws, et al. Pharm Res. Sialic Acid-Engineered IL4-10 Fusion Protein is Bioactive and Rapidly Cleared from the Circulation

[4] Cassandra Davidson, et al. Transpl Immunol. IL-13 prolongs allograft survival: association with inhibition of macrophage cytokine activation

[5] P Lu, et al. J Interferon Cytokine Res. Cloning and expression of canine interleukin-10

Related Recombinant Proteins Products
Recombinant Human ANXA2 Protein (E. coli)

Annexin A2 (ANXA2) is a member of the annexin family and has roles in the regulation of cellular growth and in signal transduction pathways.

Recombinant Human IL-17B (Mammalian, C-Fc)

Interleukin-17B (IL-17B) is a member of IL-17 cytokines family that plays important roles in host defence responses and inflammatory diseases.

Recombinant Human GOLM1 Protein(C-6His)

Golgi Membrane Protein 1 (GOLM1) belongs to the GOLM1/CASC4 family, GOLM1 is a single-pass type II membrane protein and can be found in many tissues . GOLM1 is overexpressed in prostate cancer and lung adenocarcinoma tissue. GOLM1 can be up-regulated in response to viral infection. GOLM1 is induced by the E1A adenoviral protein. GOLM1 plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.

Recombinant Mouse Apolipoprotein E (Mammalian, C-6His)

Apolipoprotein E (Apo-E) is a member of the apolipoprotein A1/A4/E family. APOE may function in mediating the binding, internalization, and catabolism of lipoprotein particles.

Recombinant Human Granzyme B Protein (HEK293, C-His)

Granzyme B is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells.

  Catalog
Abmole Inhibitor Catalog




Keywords: Recombinant Rat IL-10 (CHO), B-TCGF; CSIF; TGIF supplier, Recombinant Proteins, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.