Interleukin-10 (IL-10) is produced by many immune cells, such as T cells, macrophages, mast cells and dendritic cells. It is usually secreted as a homodimer and, upon binding to receptors, inhibits the synthesis of several cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and Th2 cells. It also displays the ability to suppress Antigen-Presenting Cell (APC) function.
Measured in a bioassay using C6 cells, the ED50 is < 8.0 μg /ml.
Protein Construction: IL-10 (Ser19-Asn178)
Accession#: P29456
Apparent Molecular Weight: 8~22 kDa, reducing conditions
Endotoxin < 0.2 EU/μg
Lyophilized after extensive dialysis against PBS.
| Form | Lyophilized powder |
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for up to 6 months after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[5] P Lu, et al. J Interferon Cytokine Res. Cloning and expression of canine interleukin-10
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