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Recombinant Murine Midkine (E. coli)

Cat. No. M10286
Recombinant Murine Midkine (E. coli) Structure
Synonym:

rmMK; rm-midkine

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5ug USD 170  USD170 In stock
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Quality Control & Documentation
  • Purity: >96%, Endotoxin <1EU/μg
  • COA
  • MSDS
Biological Activity

Midkine is a heparin-binding growth factor, which contains 121 amino acid residues with 5 disulfide bonds. Midkine promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The C-terminal portion contains the heparin-binding domain and the antigenicity and neurite-promoting sequences. Both the C- and N-terminals are necessary for the molecule′s neurotrophic effects.

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.


Expression system : E. coli

Accession: P12025

Approximately Molecular Weight: 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.

AA Sequence:
VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD

Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4.

Chemical Information
Solubility (25°C) Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
Storage Stable for up to twelve months from date of receipt at -20℃ to -70℃. Reconstituted protein solution can be stored at 2-8°C for 2 weeks, at ≤ -20°C for 3 months.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Ludwig T Weckbach, et al. Blood. The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via β2 integrins (CD11/CD18)

[2] S Kojima, et al. J Biol Chem. Midkine enhances fibrinolytic activity of bovine endothelial cells

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