Midkine is a heparin-binding growth factor, which contains 121 amino acid residues with 5 disulfide bonds. Midkine promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The C-terminal portion contains the heparin-binding domain and the antigenicity and neurite-promoting sequences. Both the C- and N-terminals are necessary for the molecule′s neurotrophic effects.
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.
Expression system : E. coli
Accession: P12025
Approximately Molecular Weight: 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.
AA Sequence:
VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4.
Solubility (25°C) | Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. |
Storage | Stable for up to twelve months from date of receipt at -20℃ to -70℃. Reconstituted protein solution can be stored at 2-8°C for 2 weeks, at ≤ -20°C for 3 months. |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[2] S Kojima, et al. J Biol Chem. Midkine enhances fibrinolytic activity of bovine endothelial cells
Related Recombinant Proteins Products |
---|
Recombinant Human Tau-F Protein (E.coli)
Tau proteins are proteins which contain four Tau/MAP repeats. They promote microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. |
Recombinant Human Tau Protein (E. coli, His Tag)
Tau proteins are proteins that stabilize microtubules. Tau protein is a highly soluble microtubule-associated protein (MAP). In humans, these proteins are mostly found in neurons compared to non-neuronal cells. One of tau's main functions is to modulate the stability of axonal microtubules. |
Recombinant Rat IL-23 Protein (HEK293, C-His)
IL-23 (IL-23 alpha & IL-12 beta Heterodimer) Protein is a recombinant protein dimer complex containing rat-derived IL-23 alpha & IL-12 beta Heterodimer protein, expressed by HEK293. |
Recombinant Rat IL-6 (HEK293)
Interleukin-6 (IL-6), also known as BSF-2, CDF and IFNB2, is a pleiotropic cytokine that participates in both pro-inflammatory and anti-inflammatory responses. |
Recombinant Rat IL-12 (Mammalian, C-6His)
Interleukin 12 (IL-12) has important immunological functions. IL12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.