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Recombinant Mouse RANKL (E.coli)

Cat. No. M15095
Recombinant Mouse RANKL (E.coli) Structure
Synonym:

TNF Ligand Superfamily Member 11

Size Price Availability Quantity
10ug USD 300  USD300 In stock
50ug USD 900  USD900 In stock
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Quality Control
  • Current batch:
  • Purity >95% , Endotoxin < 0.01 EU/µg
  • COA
  • MSDS
Biological Activity

Species: Mouse
Expression system: E.coli
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: LAL test: less than 0.001 ng/µg (0.01 EU/µg)
Storage and Stability: lyophilized proteins should be stored at < -20°C, but can be stored for up to 3 weeks at room temperature. The recombinant protein solution can be stored at 4-7 ° C for 2-7 days. Aliquots of recombinant samples were stable at < -20°C for 3 months.


Bioactivity: Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor (TNF) cytokine family. Tnfsf11 is widely expressed in cells, including T cells, and in T cell-rich organs, such as the thymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG and TNFRSF11A /RANK. Tnfsf11 is involved in many fundamental biological processes, such as acting as a regulator of interactions between T cells and dendritic cells, regulating T cell-dependent immune responses and enhancing bone resorption in malignant humoral hypercalcemia. It enhances the ability of dendritic cells to stimulate the proliferation of naive T cells.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight
Formula
CAS Number
Purity >95% , Endotoxin < 0.01 EU/µg
Solubility It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Storage at -20°C
References

[1] Luyang Che, et al. Sheng Wu Gong Cheng Xue Bao. [Enhancing hRANKL production in Escherichia coli by optimizing culture conditions and lysing program]

[2] Mengmeng Jin, et al. Appl Microbiol Biotechnol. Sortase A-aided Escherichia coli expression system for functional osteoprotegerin cysteine-rich domain

[3] Jinmoon Kim, et al. J Microbiol. Role of bacterial γ-glutamyltranspeptidase as a novel virulence factor in bone-resorbing pathogenesis

[4] Thomas S Walter, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. Crystallization and preliminary X-ray analysis of mouse RANK and its complex with RANKL

[5] Karl D Husler, et al. J Bone Miner Res. Secreted frizzled-related protein-1 inhibits RANKL-dependent osteoclast formation

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Keywords: Recombinant Mouse RANKL (E.coli), TNF Ligand Superfamily Member 11 supplier, Cytokines, inhibitors

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