Species: Mouse
Expression system: Mammalian
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: less than 0.1 ng/ug (1 EU/ug) as tested by LAL
Storage and stability: Lyophilized proteins should be stored at < -20°C but can be stored at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.
Bioactivity: Noggin is a secreted homodimer glycoprotein that is an antagonist of bone morphogenetic protein (BMP). Mouse Noggin cDNA encodes a precursor protein with 232 amino acids (AA) residues and a presumed signal peptide with 19 AA residues, cleaved to yield 213 AA residue mature proteins that are secreted as homodimer glycoproteins. Due to binding to heparin-containing proteoglycans, secreted Noggin may remain close to the cell surface. Noggin has high affinity with some PARTS of BMP such as BMP4 and low affinity with some parts of BMP such as BMP7. It antagonizes the biological activity of BMP by blocking epitopes required on BMP to bind to type I and type II receptors. Noggin is expressed in specific regions of the central nervous system and surrounding tissues such as lung, skeletal muscle and skin in adults. When human embryonic stem cells (hESC) or neural stem cells (NSCS) were cultured under certain conditions, Noggin antagonized BMP activity could induce stem cells to remain undifferentiated and proliferate or differentiate into dopaminergic neurons.
Solubility (25°C) | It is not recommended to reconstitute to a concentration less than 100 μg/ml. |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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