Species: Mouse
Expression system: E.coli
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: less than 0.1 ng/ug (1 EU/ug) as tested by LAL
Storage and stability: Lyophilized proteins should be stored at < -20°C but can be stored at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.
Bioactivity: The ability to induce the activity of the STAT reporter factor in 293F human embryonic kidney cells was determined. The ED50 of this effect was 187ng /mL. Mouse Leukemia inhibitory factor (LIF) is a secreted protein belonging to the LIF /OSM family. LIF is involved in many physiological processes, including development, hematopoiesis, bone metabolism and inflammation. It has the ability to induce terminal differentiation of leukemia cells. Its activities include inducing hematopoietic differentiation of normal and myeloid leukemia cells, inducing neuronal differentiation, and stimulating acute phase protein synthesis of hepatocytes.
Solubility (25°C) | It is not recommended to reconstitute to a concentration less than 100 μg/ml. |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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