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Recombinant Mouse LIF (E.coli)

Cat. No. M15077

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Mouse LIF (E.coli) Structure
Synonym:

Leukemia inhibitory factor;Differentiation-stimulating factor; mLIF

Size Price Availability Quantity
10ug USD 185  USD185 In stock
50ug USD 420  USD420 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 1 EU/ug
  • COA
  • MSDS
Biological Activity

Mouse Leukemia inhibitory factor (LIF) is a secreted protein belonging to the LIF /OSM family. LIF is involved in many physiological processes, including development, hematopoiesis, bone metabolism and inflammation. It has the ability to induce terminal differentiation of leukemia cells. Its activities include inducing hematopoietic differentiation of normal and myeloid leukemia cells, inducing neuronal differentiation, and stimulating acute phase protein synthesis of hepatocytes.

The ability to induce the activity of the STAT reporter factor in 293F human embryonic kidney cells was determined, the ED50 of this effect was 187 ng/mL.

Recombinant Mouse Leukemia Inhibitory Factor expressed the target gene encoding Ser24-Phe203.


Accession: P09056

Endotoxin < 1 EU/µg

Apparent Molecular Weight: 18 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
References

[1] Nahid Mehri, et al. Mol Biotechnol. Optimizing the Expression and Solubilization of an E. coli-Produced Leukemia Inhibitory Factor for Anti-LIF Antibody Production and Use Thereof for Contraception in Mice

[2] Yanan Guo, et al. Protein Expr Purif. Production of soluble bioactive mouse leukemia inhibitory factor from Escherichia coli using MBP tag

[3] Xueyan Xi, et al. Protein Expr Purif. Expression, purification and characterization of active untagged recombinant human leukemia inhibitory factor from E.coli

[4] Bradford A Youngblood, et al. J Biotechnol. Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells

[5] Hiroyuki Horiuchi, et al. J Biol Chem. Chicken leukemia inhibitory factor maintains chicken embryonic stem cells in the undifferentiated state

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Keywords: Recombinant Mouse LIF (E.coli), Leukemia inhibitory factor;Differentiation-stimulating factor; mLIF supplier, Cytokines and Growth Factors, inhibitors, activators

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