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Recombinant Mouse LIF (E.coli)

Cat. No. M15077
Recombinant Mouse LIF (E.coli) Structure
Synonym:

Leukemia inhibitory factor;Differentiation-stimulating factor; mLIF

Size Price Availability Quantity
10ug USD 185  USD185 In stock
50ug USD 420  USD420 In stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin < 1 EU/ug
  • COA
  • MSDS
Biological Activity

Species: Mouse
Expression system: E.coli
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: less than 0.1 ng/ug (1 EU/ug) as tested by LAL
Storage and stability: Lyophilized proteins should be stored at < -20°C but can be stored at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.


Bioactivity: The ability to induce the activity of the STAT reporter factor in 293F human embryonic kidney cells was determined. The ED50 of this effect was 187ng /mL. Mouse Leukemia inhibitory factor (LIF) is a secreted protein belonging to the LIF /OSM family. LIF is involved in many physiological processes, including development, hematopoiesis, bone metabolism and inflammation. It has the ability to induce terminal differentiation of leukemia cells. Its activities include inducing hematopoietic differentiation of normal and myeloid leukemia cells, inducing neuronal differentiation, and stimulating acute phase protein synthesis of hepatocytes.

Chemical Information
Solubility (25°C) It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Nahid Mehri, et al. Mol Biotechnol. Optimizing the Expression and Solubilization of an E. coli-Produced Leukemia Inhibitory Factor for Anti-LIF Antibody Production and Use Thereof for Contraception in Mice

[2] Yanan Guo, et al. Protein Expr Purif. Production of soluble bioactive mouse leukemia inhibitory factor from Escherichia coli using MBP tag

[3] Xueyan Xi, et al. Protein Expr Purif. Expression, purification and characterization of active untagged recombinant human leukemia inhibitory factor from E.coli

[4] Bradford A Youngblood, et al. J Biotechnol. Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells

[5] Hiroyuki Horiuchi, et al. J Biol Chem. Chicken leukemia inhibitory factor maintains chicken embryonic stem cells in the undifferentiated state

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Keywords: Recombinant Mouse LIF (E.coli), Leukemia inhibitory factor;Differentiation-stimulating factor; mLIF supplier, Recombinant Proteins, inhibitors, activators


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