Genus: Mouse
Expression system: E. coli
Purity: >95% by SDS-PAGE analysis
Endotoxin: < 0.2 EU/μg of protein by gel coagulation
Apparent molecular weight: ~15.2 kDa on SDS-PAGE under non-reducing conditions
Storage and Stability: This product is stable for up to 6 months at temperatures below -70°C after receipt. After reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long-term storage, the addition of a carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: Interleukin-3 (IL-3) is a polymorphic cytokine belonging to the interleukin family.IL-3 shares similarities with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-5: they both have a four-helix bundle structure, are located on the same chromosome in both humans and mice, are produced by activated T cells, and have a common receptor.IL- IL-3 is also known as a multicolony stimulating factor because it stimulates the development and colony formation of multilineage hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. -2 gene family to inhibit apoptosis and promote cell survival.
| Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[4] A Kusumi, et al. Gan To Kagaku Ryoho. [Interleukin 3 (IL-3)]
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