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Recombinant Mouse IL-2 (E.coli)

Cat. No. M19999
Recombinant Mouse IL-2 (E.coli) Structure
Synonym:

aldesleukin; interleukin 2; interleukin-2

Size Price Availability Quantity
10ug USD 155  USD155 In stock
50ug USD 460  USD460 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.01 EU/µg
  • COA
  • MSDS
Biological Activity

Interleukin 2 (IL 2), also known as T-cell growth factor, is a member of a family of cytokines that includes IL-4, IL-7, IL-9, IL-15 and IL-21. IL-2 signaling is transmitted through the IL-2 receptor, a complex of tree-like subunits called alpha, beta, and gamma. IL-2 is produced by CD4+ T cells, CD8+ T cells, γδT cells, B cells, dendritic cells and eosinophils, and plays an important role in key functions of the immune system, tolerance and immunity, mainly through its powerful stimulatory activity on T cells. Thus, IL-2 may be a key cytokine that naturally suppresses autoimmunity.

Recombinant Mouse Interleukin-2 is produced by E.coli expression system, and expressed the target gene encoding Ala21-Gln169.

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells, the specific activity of Recombinant Mouse IL-2 is ≥1×107 IU/mg.


Accession: P04351

Endotoxin < 0.01 EU/µg

Apparent Molecular Weight: 17 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of 20mM Sodium Citrate, 0.2% Tween 80, pH 3.0.

Product Citations
Chemical Information
Form Lyophilized powder
Solubility (25°C) Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
Storage Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Jianlin Chen, et al. Biology (Basel). Co-Expression of Pig IL-2 and Fusion Bovine Cathelicidin Gene by Recombinant Plasmids in Yeast and Their Promotion of Mouse Antibacterial Defense

[2] Elham Adabi, et al. Iran Biomed J. Evaluation of an Albumin-Binding Domain Protein Fused to Recombinant Human IL-2 and Its Effects on the Bioactivity and Serum Half-Life of the Cytokine

[3] Can Xu, et al. World J Gastroenterol. Construction of recombinant attenuated Salmonella typhimurium DNA vaccine expressing H pylori ureB and IL-2

[4] V Bronte, et al. J Immunol. IL-2 enhances the function of recombinant poxvirus-based vaccines in the treatment of established pulmonary metastases

[5] C M Roifman, et al. Cell Immunol. Functional comparison of recombinant interleukin 2 (IL-2) with IL-2-containing preparations derived from cultured cells

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Keywords: Recombinant Mouse IL-2 (E.coli), aldesleukin; interleukin 2; interleukin-2 supplier, Recombinant Proteins, inhibitors, activators


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