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Recombinant Mouse IL-1β (CHO)

Cat. No. M25418
Recombinant Mouse IL-1β (CHO) Structure
Synonym:

interleukin 1 beta

Size Price Availability Quantity
10ug USD 200  USD200 In stock
50ug USD 550  USD550 In stock
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Quality Control & Documentation
  • Purity: >95%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Interleukin-1 Beta (IL-1β) (E. coli) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. ED50 < 10.0 pg/ml, measured in a cell proliferation assay using D10S cells, corresponding to a specific activity of > 1.0 × 108 units/mg.


Accession #: P10749

Apparent Molecular Weight: ~17.4 kDa, on SDS-PAGE under non-reducing conditions.

Endotoxin Level: < 0.2 EU/μg of protein by gel clotting method

Lyophilized after extensive dialysis against PBS.

Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage Stable for up to 6 months at ≤ -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C.
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Megumi Mae, et al. Int J Mol Sci. The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis

[2] Hyun Seung Lee, et al. Exp Mol Med. Role of interleukin-23 in the development of nonallergic eosinophilic inflammation in a murine model of asthma

[3] Shen-An Hwang, et al. Innate Immun. Effects of CHO-expressed recombinant lactoferrins on mouse dendritic cell presentation and function

[4] Ki-Hong Kim, et al. J Immunol Methods. Interleukin-32 monoclonal antibodies for immunohistochemistry, Western blotting, and ELISA

[5] Mi-La Cho, et al. J Immunol. STAT3 and NF-kappaB signal pathway is required for IL-23-mediated IL-17 production in spontaneous arthritis animal model IL-1 receptor antagonist-deficient mice

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Keywords: Recombinant Mouse IL-1β (CHO), interleukin 1 beta supplier, Recombinant Proteins, inhibitors, activators


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