GSK-3 beta/GSK3B Protein is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB, and the transcription factor JUN. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which are critical for its role in bulge stem cell migration and skin wound repair.
The recombinant mouse GSK3B consists of 439 amino acids and has a calculated molecular mass of 49 kDa. It expressed the target gene encoding the mouse GSK3B (Met 1-Thr 420) with a polyhistidine tag at the N-terminus.
Immobilized His-mGSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.
Endotoxin < 1 EU/µg
Apparent Molecular Weight: 47 kDa, reducing conditions
Supplied as sterile 20mM Tris, 500mM NaCl, 25% glycerol, 0.2mM DTT, pH 7.4.
Shipping with dry ice.
| Form | Solution |
| Storage | Stable for up to twelve months from date of receipt at -20℃ to -80℃. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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