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Recombinant Mouse FGF-9 (E. coli)

Cat. No. M15031

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Mouse FGF-9 (E. coli) Structure
Synonym:

Fibroblast Growth Factor-9; FGF9

Size Price Availability Quantity
10ug USD 205  USD205 In stock
50ug USD 455  USD455 In stock
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Quality Control & Documentation
  • Purity: >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Species: Mouse
Expression system: E. coli
Purity: SDS-PAGE analysis, > 95%, HIGH performance liquid chromatography analysis, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: ~23.4 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.


Bioactivity: ED50 < 5.0 ng/ mL, determined by 3T3 cell proliferation method, the corresponding specific activity was > 2.0 × 105 units /mg. Reversible dimerization of FGF-9 is a common feature of its subfamily members FGF-16 and FGF-20. Mutations involved in homologous dimerization also affect heparin affinity, FGF receptor binding, and biological activity. In vivo, FGF-9 is expressed in limb buds, developing bone, and the gut during late embryogenesis. Fgf-9 is critical for the development of the heart, lungs, kidneys, cecum and testes; Reduced FGF-9 levels lead to early differentiation. Fgf-9 also promotes nephron progenitor cell survival in conjunction with bone morphogenetic protein 7 (BMP-7).

Chemical Information
Solubility (25°C) It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
References

[1] Shen Wang, et al. Appl Microbiol Biotechnol. Expression and purification of an FGF9 fusion protein in E. coli, and the effects of the FGF9 subfamily on human hepatocellular carcinoma cell proliferation and migration

[2] S Santos-Ocampo, et al. J Biol Chem. Expression and biological activity of mouse fibroblast growth factor-9

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Keywords: Recombinant Mouse FGF-9 (E. coli), Fibroblast Growth Factor-9; FGF9 supplier, Cytokines and Growth Factors, inhibitors, activators

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