Species: Mouse
Expression system: E.coli
Purity: > 95% as determined by reduced SDS-PAGE
Endotoxin: less than 0.001 ng/ug (0.01 EU/ug) as determined by LAL
Storage and stability: Lyophilized proteins should be stored at < -20°C but can be stored at room temperature for up to 3 weeks. The recombinant protein solution can be stored at 4-7℃ for 2-7 days. The isometric solution of the recombinant sample was stable at < -20°C for 3 months.
Bioactivity: C-C Motif chemokine 2 (CCL2) is a member of the C-C or β chemokine family. The amino acid (AA) homology of mouse CCL2 was 82% with rat CCL2, and 58%, 56%, 55%, 53% and 53% with human, horse, pig, cattle and dog CCL2, respectively. Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can form or produce CCL2 in response to mitotic stimulation, but mononuclear cells and macrophages seem to be the main sources. In addition to its chemotactic activity, CCL2 induces monocytes, NK cells, and lymphocytes to release enzymes and cytokines, and induces histamine release from basophils expressing their receptor CCR2. In addition, it promotes Th2 polarization of CD4+ T cells. Ccl2-mediated recruitment of monocytes to inflammatory sites is thought to play a role in the pathology of atherosclerosis, multiple sclerosis, and allergic asthma.
| Solubility (25°C) | It is not recommended to reconstitute to a concentration less than 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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