Species: Rat
Expression system: HEK 293
Purity: 98% by SDS-PAGE analysis
Endotoxin: Protein < 0.2 EU/μg measured by gel coagulation
Apparent molecular weight: ~8.2 kDa, reduction conditions on SDS-PAGE
Storage and Stability: After receipt, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stabilized at 4°C for 1 week or at -20°C for 3 months. For long-term preservation, carrier protein (e.g., 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: The EC50 value of rat MIP-3α /CCL20 for Ca2+ mobilization in CHO-K1/ G15/rCCR6 cells (human G15 and rat CCR6 are stably expressed in CHO-K1 cells) was less than 0.6 μg/ml. Mip-3α is expressed in liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. It has a strong chemotactic effect on lymphocytes and a weak attractive effect on neutrophils. Mip-3α is involved in the formation and function of mucosal lymphoid tissue through the attraction of lymphocytes and dendritic cells to the epithelial cells surrounding mucosal lymphoid tissue. Promote memory CD4+ T cell adhesion and inhibit bone marrow immature progenitor cell colony formation.
Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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