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Recombinant Rat MIP-3 α/CCL20 (HEK 293)

Cat. No. M19981

All AbMole products are for research use only, cannot be used for human consumption.

Recombinant Rat MIP-3 α/CCL20 (HEK 293) Structure
Synonym:

CCL-20; CKb4; Exodus; LARC; MIP-3-alpha

Size Price Availability Quantity
5ug USD 150  USD150 In stock
25ug USD 250  USD250 In stock
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Quality Control & Documentation
  • Purity: >98% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
Biological Activity

Species: Rat
Expression system: HEK 293
Purity: 98% by SDS-PAGE analysis
Endotoxin: Protein < 0.2 EU/μg measured by gel coagulation
Apparent molecular weight: ~8.2 kDa, reduction conditions on SDS-PAGE
Storage and Stability: After receipt, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stabilized at 4°C for 1 week or at -20°C for 3 months. For long-term preservation, carrier protein (e.g., 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.

Bioactivity: The EC50 value of rat MIP-3α /CCL20 for Ca2+ mobilization in CHO-K1/ G15/rCCR6 cells (human G15 and rat CCR6 are stably expressed in CHO-K1 cells) was less than 0.6 μg/ml. Mip-3α is expressed in liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. It has a strong chemotactic effect on lymphocytes and a weak attractive effect on neutrophils. Mip-3α is involved in the formation and function of mucosal lymphoid tissue through the attraction of lymphocytes and dendritic cells to the epithelial cells surrounding mucosal lymphoid tissue. Promote memory CD4+ T cell adhesion and inhibit bone marrow immature progenitor cell colony formation.

Chemical Information
Solubility (25°C) It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
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Keywords: Recombinant Rat MIP-3 α/CCL20 (HEK 293), CCL-20; CKb4; Exodus; LARC; MIP-3-alpha supplier, Cytokines and Growth Factors, inhibitors, activators

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