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Recombinant Human VWF Protein (CHO, His Tag)

Cat. No. M25480
Recombinant Human VWF Protein (CHO, His Tag) Structure
Synonym:

Von Willebrand Factor; rhvwf

Size Price Availability Quantity
100ug USD 1000  USD1000 In stock
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Quality Control & Documentation
  • Purity: >75%, Endotoxin < 1 EU/µg
  • COA
  • MSDS
Biological Activity

Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.

A DNA sequence encoding the pro form of human Von Willebrand factor (AAB59458.1) (Met1-Lys2813) was expressed with a C-terminal polyhistidine tag.

The recombinant human VWF consists of 2802 amino acids after removal of the signal peptide and has a predicted molecular mass of 308 kDa.


Endotoxin: < 1.0 EU/μg

Lyophilized from sterile PBS, pH 7.4.

Chemical Information
Solubility (25°C) Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage Stable for up to twelve months from date of receipt at -20℃ to -80℃
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Juan A De Pablo-Moreno, et al. Int J Mol Sci. The Vascular Endothelium and Coagulation: Homeostasis, Disease, and Treatment, with a Focus on the Von Willebrand Factor and Factors VIII and V

[2] Ruchika Sharma, et al. Hematology Am Soc Hematol Educ Program. New advances in the diagnosis of von Willebrand disease

[3] Meaghan E Colling, et al. Clin Appl Thromb Hemost. In Vitro Assessment of von Willebrand Factor in Cryoprecipitate, Antihemophilic Factor/VWF Complex (Human), and Recombinant von Willebrand Factor

[4] Giancarlo Castaman, et al. Eur J Haematol. Pregnancy and delivery in women with von Willebrand disease

[5] Eva-Maria Muchitsch, et al. Semin Thromb Hemost. Preclinical testing of human recombinant von Willebrand factor: ADAMTS13 cleavage capacity in animals as criterion for species suitability

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Keywords: Recombinant Human VWF Protein (CHO, His Tag), Von Willebrand Factor; rhvwf supplier, Recombinant Proteins, inhibitors, activators


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